Dynamics of Viral Load in Plasma and HIV DNA in Lymphocytes During Highly Active Antiretroviral Therapy (HAART): High Viral Burden in Macrophages after 1 Year of Treatment
| Autor: | Arrigo Benedetto, M. Zaccarelli, Anna Rosa Garbuglia, Giuseppina Cappiello, S. Calcaterra, R. Marini |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Lymphocyte HIV Infections Polymerase Chain Reaction Virus Acquired immunodeficiency syndrome (AIDS) Antiretroviral Therapy Highly Active medicine Humans Pharmacology (medical) Protease inhibitor (pharmacology) Lymphocytes Disease Reservoirs Pharmacology biology Reverse-transcriptase inhibitor Macrophages virus diseases Viral Load biology.organism_classification medicine.disease Virology CD4 Lymphocyte Count Infectious Diseases medicine.anatomical_structure Oncology DNA Viral Lentivirus Immunology RNA Viral Female Viral disease Viral load Follow-Up Studies medicine.drug |
| Zdroj: | Journal of Chemotherapy. 13:188-194 |
| ISSN: | 1973-9478 1120-009X |
| DOI: | 10.1179/joc.2001.13.2.188 |
| Popis: | In this study we evaluated the level of HIV RNA in plasma and HIV DNA in peripheral blood cells. Sixteen antiretroviral-experienced HIV patients with severe immune suppression were included in the study. After the first month, 56.2% of the patients showed undetectable levels of HIV RNA, this percentage remaining stable after 1 year (53.3%). At enrollment, 7 patients (43.7%) with a low CD4+ T cell count (mean 22 per mm3 versus 73) showed HIV DNA levels below the limit of detection (5 copies/10(5)) in lymphocytes. They all subsequently had increased HIV DNA that became detectable between the first and the third month of therapy, associated with an increase of the CD4+ T cell count (mean 22 to 95/mm3); in 4 of these patients this increase was transitory, becoming undetectable again after one year. In 7 out of the 8 patients with detectable HIV DNA at enrollment, the HIV DNA level decreased over time. Seven out of 15 patients at 1 year (46.7%) showed both undetectable levels of HIV RNA in plasma and HIV DNA in lymphocytes (p0.05); these patients had a higher CD4+ T cell count at baseline (mean 75 versus 25/mm3) and a higher increase (306 versus 177/mm3) after 1 year. PCR-based dilution assay carried out at 1 year showed that all patients had a consistent amount of HIV DNA positive- CD4+ T lymphocytes and macrophages, with higher values in these last cells. The data indicate that a durable reservoir of virus is still present in both lymphocytes and monocytes, even after long-lasting HAART treatment. |
| Databáze: | OpenAIRE |
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