Use of ATP, dATP and their α-thio derivatives to study DNA ligase adenylation
| Autor: | Alessandra Montecucco, Guido Pedrali-Noy, Silvio Spadari, Giovanni Ciarrocchi, Marta Lestingi |
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| Rok vydání: | 1990 |
| Předmět: |
DNA Ligases
Reversion Thio Biology Biochemistry Phosphates Adenosine Triphosphate Deoxyadenine Nucleotides Humans heterocyclic compounds Molecular Biology Adenylylation chemistry.chemical_classification DNA ligase Antibiotics Antineoplastic Collodion Cell Biology Thionucleotides Enzyme chemistry Covalent bond T-Phages Ligation Research Article HeLa Cells |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj2710265 |
| Popis: | Bacteriophage-T4 and human type I DNA ligases were found capable of self-adenylating upon exposure to both ribo- and deoxyribo-[alpha-35S]thio-ATP. However, the joining reaction does not take place in the presence of the deoxyribotriphosphates. Enzyme adenylation is reversed in all cases by an excess of PPi, but the rate of reversion is lower with thio derivatives. Therefore thio derivatives can be used to study the adenylation of DNA ligases and to search for specific inhibitors of the first step of the ligation reaction. In addition we show that thio derivatives can be used to detect DNA ligase adenylation activity covalently bound to a solid matrix. |
| Databáze: | OpenAIRE |
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