beta-Amyloid precursor protein fragments and lysosomal dense bodies are found in rat brain neurons after ventricular infusion of leupeptin
| Autor: | V.E.R. Anderson, C. C. Nolan, M. P. Seville, Iradj Hajimohammadreza, J. B. Cavanagh, Peter Leigh, B.H. Anderton |
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| Rok vydání: | 1994 |
| Předmět: |
Leupeptins
Blotting Western Hippocampal formation Cytoplasmic Granules chemistry.chemical_compound Amyloid beta-Protein Precursor Ubiquitin Lysosome mental disorders medicine Extracellular Amyloid precursor protein Animals Rats Wistar Molecular Biology Injections Intraventricular Neurons biology General Neuroscience Leupeptin Antibodies Monoclonal Brain Dendrites Immunohistochemistry Axons Peptide Fragments Cell biology Rats medicine.anatomical_structure nervous system Biochemistry chemistry Cytoplasm biology.protein Female Neurology (clinical) Neuron Lysosomes Developmental Biology |
| Zdroj: | Brain research. 640(1-2) |
| ISSN: | 0006-8993 |
| Popis: | Infusion of the serine and thiol protease inhibitor, leupeptin, is known to cause a reduction of fast axoplasmic transport, and accumulation of lysosomal dense bodies in neuronal perikarya. We have found these dense bodies in hippocampal and cerebellar neurons contain ubiquitin conjugated proteins. We now demonstrate that these accumulated neuronal lysosomes are labeled by antisera to the cytoplasmic, transmembrane and extracellular domains of β-amyloid precursor protein (APP) and also that lysosomal APP is fragmented. This in vivo model confirms that neurons can process APP via a lysosomal pathway and that neuronal lysosomes in vivo contain both N-terminal and potentially amyloidogenic C-terminal fragments of APP. We also show that increased APP immunoreactivity after leupeptin treatment is seen first in neurons and later in astrocytes. On recovery from infusion, APP N-terminal immunoreactivity diminishes whilst C-terminal reactivity remains in neurons. These findings are consistent with production in whole brain of potentially amyloidogenic fragments of APP within neuronal lysosomes in perikarya and dendrites implying that neurons may play a role in forming the β-amyloid of plaques. |
| Databáze: | OpenAIRE |
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