Human Dendritic Cells Respond to Helicobacter pylori, Promoting NK Cell and Th1-Effector Responses In Vitro
| Autor: | Markus Gerhard, Roland Rad, Petra Voland, S. Schwendy, Nadia Hafsi, Wolfgang Reindl, Christian Prinz |
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| Rok vydání: | 2004 |
| Předmět: |
T-Lymphocytes
Immunology chemical and pharmacologic phenomena GATA3 Transcription Factor In Vitro Techniques Polymerase Chain Reaction Helicobacter Infections Microbiology Interleukin 21 Humans Immunology and Allergy Secretion CD86 MHC class II Innate immune system Helicobacter pylori biology Cell Differentiation Dendritic Cells Th1 Cells Flow Cytometry Acquired immune system Cell biology DNA-Binding Proteins Killer Cells Natural Trans-Activators biology.protein Interleukin 12 T-Box Domain Proteins CD80 Transcription Factors |
| Zdroj: | The Journal of Immunology. 173:1249-1257 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.173.2.1249 |
| Popis: | Helicobacter pylori infection leads to chronic gastric inflammation. The current study determined the response of human APCs, NK cells, and T cells toward the bacteria in vitro. Human monocyte-derived dendritic cells (DC) were incubated with bacteria for 48 h. Intact H. pylori at a multitude of infection 5 stimulated the expression of MHC class II (4- to 7-fold), CD80, and CD86 B7 molecules (10- to 12-fold) and the CD83 costimulatory molecule (>30-fold) as well as IL-12 secretion (>50-fold) in DCs, and thereby, strongly induced their maturation and activation. CD56+/CD4− NK cells, as well as CD4+/CD45RA+ naive T cells, were isolated and incubated with DCs pulsed with intact bacteria or different cellular fractions. Coculture of H. pylori-pulsed DCs with NK cells strongly potentiated the secretion of TNF-α and IFN-γ. Coculture of naive T cells with H. pylori-pulsed DCs significantly enhanced TNF-α, IFN-γ, and IL-2 secretion as well as T-bet mRNA levels, while GATA-3 mRNA was lowered. However, the effect appeared attenuated compared with coculture with Escherichia coli. A greater stimulation was seen with naive T cells and DCs pulsed with H. pylori membrane preparations. Intact H. pylori potently induced the maturation and activation of human monocyte-derived DC and thereby promote NK and Th1 effector responses. The strong activation of NK cells may be important for the innate immune response. Th1-polarized T cells were induced especially by incubation with membrane preparations of H. pylori, suggesting that membrane proteins may account for the specific adaptive immune response. |
| Databáze: | OpenAIRE |
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