An Attempt to Make Platelet Rich Fibrin from Bone Marrow Aspirate
| Autor: | Hisako Ishise, Masao Kakibuchi, Kenichiro Kawai, Kazutoshi Fujita, Toshihiro Fujiwara, Maki Tonooka, Yohei Sotsuka, Soh Nishimoto |
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| Rok vydání: | 2017 |
| Předmět: |
Pathology
medicine.medical_specialty bm-prf Clinical Biochemistry lcsh:Medicine chemistry.chemical_element Surgery Section 030230 surgery Calcium Iliac crest komiya's bone marrow needle 03 medical and health sciences 0302 clinical medicine Nucleated cell medicine Platelet Centrifugation business.industry anti-coagulant lcsh:R 030206 dentistry General Medicine Haemolysis nucleated cell Platelet-rich fibrin yellow-red interface medicine.anatomical_structure chemistry Bone marrow business |
| Zdroj: | Journal of Clinical and Diagnostic Research, Vol 11, Iss 8, Pp PC04-PC07 (2017) |
| ISSN: | 2249-782X |
| Popis: | Introduction Platelet Rich Fibrin (PRF) was developed as a new generation of platelet concentration from peripheral blood. Method to make PRF is simple. It is easy to handle with its moderate firmness. Histologically, platelets and nucleated cells are packed along the yellow-red border. Bone marrow aspirate contains bone marrow cells that potentially work for tissue regeneration, and platelets which contain growth factors. The specific gravities of them are comparable. It implies that, if it is possible to make PRF from bone marrow aspirate, then high concentration of platelets and bone marrow cells can be obtained simultaneously by taking out yellow-red interface of it. Aim To find out a method to make PRF from bone marrow aspirate. Materials and methods Iliac crest of rabbits were punctured and aspirated with or without anti-coagulant, under general anaesthesia. The bone marrow aspirate was centrifuged in glass tubes. For the bone marrow aspirate taken with anti-coagulant, calcium chloride was added just before centrifugation. Products were taken out and observed grossly. The products were fixed with formaldehyde and observed histologically. Results Coagulated gels with two-toned colour were obtained by all methods. In the gels without anti-coagulant, interfaces between two colours were obscure. Histologically, platelets and nucleated cells scattered as clusters. Filtering caused haemolysis and reduced the yield of the product. With the aspirate taken with anti-coagulant, platelets and nucleated cells formed a band along the interface. Conclusion PRF can be made from bone marrow aspirate by adding anti-coagulant in aspiration and reversed with calcium chloride just before centrifugation. |
| Databáze: | OpenAIRE |
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