Exosomes-derived miR-154-5p attenuates esophageal squamous cell carcinoma progression and angiogenesis by targeting kinesin family member 14

Autor:	Yuwei, Shou, Xiaoqian, Wang, Yinghao, Liang, Xiaonan, Liu, Kuisheng, Chen
Rok vydání:	2022
Předmět:	Male Oncogene Proteins Esophageal Neoplasms Neovascularization Pathologic Kinesins Bioengineering General Medicine Middle Aged Exosomes Applied Microbiology and Biotechnology MicroRNAs Biomarkers Tumor Disease Progression Humans Female Esophageal Squamous Cell Carcinoma Biotechnology
Zdroj:	Bioengineered. 13:4610-4620
ISSN:	2165-5987 2165-5979
DOI:	10.1080/21655979.2022.2037322
Popis:	Exosomes participate in the progression and angiogenesis of esophageal squamous cell carcinoma (ESCC). This study aimed to explore the effect and mechanism of exosomes-derived miR-154-5p on the progression and angiogenesis of ESCC. The exosomes with the diameter of 40-270 nm were successfully isolated from ESCC cells by ultracentrifugation. They were then assessed by transmission electron microscope (TEM), nanoparticle tracking analysis (NTA), and Western blotting. Kinesin family member 14 (KIF14) was upregulated, while miR-154-5p was downregulated in ESCC as examined by Quantitative Real-time PCR (qRT-PCR). Exosomes-derived miR-154-5p from ESCC cells was found to attenuate the cellular migration, invasion, and angiogenesis of ESCC using Cell Counting Kit-8 (CCK-8), wound healing assay, transwell migration assay, and tumor formation assays. Moreover, KIF14 was proven to be a direct downstream target gene of miR-154-5p in ESCC cells using luciferase assay. In conclusion, our study identified that exosomes-derived miR-154-5p attenuates ESCC progression and angiogenesis by targeting KIF14
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d51ee411c366affb205c10d8e16e4031 https://doi.org/10.1080/21655979.2022.2037322 Zobrazit plný text záznamu