Self-Assembly and Biogenesis of the Cellular Membrane are Dictated by Membrane Stretch and Composition
Autor: | Akshata R. Naik, Kenneth T. Lewis, Bhanu P. Jena, Eric R. Kuhn, Douglas J. Taatjes, Jeffrey J. Potoff, Xuequn Chen, Keith M. Kokotovich, Krishna Rao Maddipati, J. H. K. Hörber |
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Rok vydání: | 2019 |
Předmět: |
Erythrocytes
Cell division Proteome Molecular Dynamics Simulation 010402 general chemistry 01 natural sciences Cell membrane Rats Sprague-Dawley Membrane Lipids Mice Cytosol 0103 physical sciences Organelle Materials Chemistry medicine Tumor Cells Cultured Animals Secretion Physical and Theoretical Chemistry Ion transporter 010304 chemical physics Chemistry Cell Membrane Blood Proteins 0104 chemical sciences Surfaces Coatings and Films Rats Pancreatic Neoplasms Membrane medicine.anatomical_structure Biophysics Tonicity lipids (amino acids peptides and proteins) Insulinoma |
Zdroj: | The journal of physical chemistry. B. 123(32) |
ISSN: | 1520-5207 |
Popis: | The cell plasma membrane is a highly dynamic organelle governing a wide range of cellular activities including ion transport, secretion, cell division, growth, and development. The fundamental process involved in the addition of new membranes to pre-existing plasma membranes, however, is unclear. Here, we report, using biophysical, morphological, biochemical, and molecular dynamic simulations, the selective incorporation of proteins and lipids from the cytosol into the cell plasma membrane dictated by membrane stretch and composition. Stretching of the cell membrane as a consequence of volume increase following incubation in a hypotonic solution and results in the incorporation of cytosolic proteins and lipids into the existing plasma membrane. Molecular dynamic simulations further confirm that increased membrane stretch results in the rapid insertion of lipids into the existing plasma membrane. Similarly, depletion of cholesterol from the cell plasma membrane selectively alters the incorporation of lipids into the membrane. |
Databáze: | OpenAIRE |
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