Anxiolytic activity of paraoxon is associated with alterations in rat brain glutamatergic system
Autor: | Zohreh Zare, Noorollah Rezaei, Moslem Mohammadi, Mohsen Tehrani, Babak Dana Ghalebarzand |
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Rok vydání: | 2019 |
Předmět: |
Male
medicine.medical_specialty Elevated plus maze medicine.drug_class Prefrontal Cortex Anxiety Motor Activity 010501 environmental sciences Toxicology Hippocampus 01 natural sciences Anxiolytic Paraoxon Open field Glutamate Plasma Membrane Transport Proteins 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine Developmental Neuroscience Internal medicine medicine Animals Cholinesterases Hippocampus (mythology) Maze Learning Prefrontal cortex 0105 earth and related environmental sciences Cholinesterase Behavior Animal Dose-Response Relationship Drug biology Chemistry Organophosphate Brain Rats Excitatory Amino Acid Transporter 1 Endocrinology Excitatory Amino Acid Transporter 2 biology.protein 030217 neurology & neurosurgery medicine.drug |
Zdroj: | Neurotoxicology and Teratology. 71:32-40 |
ISSN: | 0892-0362 |
Popis: | Exposure to organophosphate (OP) compounds leads to behavioral alterations. To determine whether paraoxon has effects on anxiety, anxiety-like behaviors were assessed in paraoxon-exposed rats. Protein expression of glutamate transporters has also been measured in hippocampus and prefrontal cortex. Three doses of paraoxon (0.3, 0.7, or 1 mg/kg) or corn oil (vehicle) were intraperitoneally injected to adult male rats. At 14 or 28 days after exposure, behavioral tests were done using elevated plus-maze (EPM) or open field tests. Thereafter, animals were sacrificed and both hippocampi and prefrontal cortices were extracted for cholinesterase assay and western blotting. Animals treated with convulsive doses of paraoxon (0.7 and 1 mg/kg) showed an increase in percentage of time spent in open arms and percentage of open arm entries in the EPM. In the open field test, an increase in the time spent in central area was observed in rats treated with the same doses of paraoxon. These effects of paraoxon were independent of any changes in locomotor activity. There was an increase in both astrocytic glutamate transporter proteins (GLAST and GLT-1) in the hippocampus of animals treated with 0.7 and 1 mg/kg of paraoxon. In the prefrontal cortex, protein levels of the GLAST and GLT-1 increased in 0.7 and decreased in 1 mg/kg groups. Only a significant decrease in EAAC1 protein was observed in the prefrontal cortex at 14 days following exposure to 1 mg/kg of paraoxon. Collectively, this study showed that exposure to convulsive doses of paraoxon induced anxiolytic-like behaviors in both behavioral tests. This effect may be attributed to alterations of glutamate transporter proteins in the rat hippocampus and prefrontal cortex. |
Databáze: | OpenAIRE |
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