Anxiolytic activity of paraoxon is associated with alterations in rat brain glutamatergic system

Autor: Zohreh Zare, Noorollah Rezaei, Moslem Mohammadi, Mohsen Tehrani, Babak Dana Ghalebarzand
Rok vydání: 2019
Předmět:
Male
medicine.medical_specialty
Elevated plus maze
medicine.drug_class
Prefrontal Cortex
Anxiety
Motor Activity
010501 environmental sciences
Toxicology
Hippocampus
01 natural sciences
Anxiolytic
Paraoxon
Open field
Glutamate Plasma Membrane Transport Proteins
03 medical and health sciences
Cellular and Molecular Neuroscience
chemistry.chemical_compound
0302 clinical medicine
Developmental Neuroscience
Internal medicine
medicine
Animals
Cholinesterases
Hippocampus (mythology)
Maze Learning
Prefrontal cortex
0105 earth and related environmental sciences
Cholinesterase
Behavior
Animal
Dose-Response Relationship
Drug
biology
Chemistry
Organophosphate
Brain
Rats
Excitatory Amino Acid Transporter 1
Endocrinology
Excitatory Amino Acid Transporter 2
biology.protein
030217 neurology & neurosurgery
medicine.drug
Zdroj: Neurotoxicology and Teratology. 71:32-40
ISSN: 0892-0362
Popis: Exposure to organophosphate (OP) compounds leads to behavioral alterations. To determine whether paraoxon has effects on anxiety, anxiety-like behaviors were assessed in paraoxon-exposed rats. Protein expression of glutamate transporters has also been measured in hippocampus and prefrontal cortex. Three doses of paraoxon (0.3, 0.7, or 1 mg/kg) or corn oil (vehicle) were intraperitoneally injected to adult male rats. At 14 or 28 days after exposure, behavioral tests were done using elevated plus-maze (EPM) or open field tests. Thereafter, animals were sacrificed and both hippocampi and prefrontal cortices were extracted for cholinesterase assay and western blotting. Animals treated with convulsive doses of paraoxon (0.7 and 1 mg/kg) showed an increase in percentage of time spent in open arms and percentage of open arm entries in the EPM. In the open field test, an increase in the time spent in central area was observed in rats treated with the same doses of paraoxon. These effects of paraoxon were independent of any changes in locomotor activity. There was an increase in both astrocytic glutamate transporter proteins (GLAST and GLT-1) in the hippocampus of animals treated with 0.7 and 1 mg/kg of paraoxon. In the prefrontal cortex, protein levels of the GLAST and GLT-1 increased in 0.7 and decreased in 1 mg/kg groups. Only a significant decrease in EAAC1 protein was observed in the prefrontal cortex at 14 days following exposure to 1 mg/kg of paraoxon. Collectively, this study showed that exposure to convulsive doses of paraoxon induced anxiolytic-like behaviors in both behavioral tests. This effect may be attributed to alterations of glutamate transporter proteins in the rat hippocampus and prefrontal cortex.
Databáze: OpenAIRE
Externí odkaz: