Two-Photon Probes for Golgi Apparatus: Detection of Golgi Apparatus in Live Tissue by Two-Photon Microscopy
| Autor: | Man So Han, Kyu Cheol Paik, Mun Seok Kim, Bong Rae Cho, Seung Taek Hong, Ji-Woo Choi |
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| Rok vydání: | 2019 |
| Předmět: |
Benzoxazoles
Photons Pyrazine Chemistry 010401 analytical chemistry Golgi Apparatus Apoptosis Golgi apparatus 010402 general chemistry 01 natural sciences 0104 chemical sciences Analytical Chemistry Rats Sprague-Dawley symbols.namesake chemistry.chemical_compound Drug Stability Microscopy Fluorescence Two-photon excitation microscopy Biophysics symbols Animals Humans Oligopeptides Fluorescent Dyes HeLa Cells |
| Zdroj: | Analytical Chemistry. 91:6669-6674 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.9b00607 |
| Popis: | We have developed blue- and yellow-emitting two-photon probes (BGolgi-blue and PGolgi-yellow) from 6-(benzo[ d]oxazol-2-yl)-2-naphthalylamine and 2,5-bis(benzo[ d]oxazol-2-yl)pyrazine derivatives as the fluorophores and trans-Golgi-network peptide (SDYQRL) as the Golgi-apparatus-targeting moiety. HeLa cells labeled with BGolgi-blue and PGolgi-yellow emitted two-photon-excited fluorescence at 462 and 560 nm, respectively, with effective two-photon-action cross-section values of 1860 and 1600 × 10 |
| Databáze: | OpenAIRE |
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