Poly (D,L-lactic-co-glycolide) Nanoparticles for the Improved Therapeutic Efficacy of All-trans-retinoic Acid: A Study of Acute Myeloid Leukemia (AML) Cell Differentiation In Vitro
| Autor: | Emimol Abraham, Nisha Asok Kumar, Aswathy Mary Simon, Asha Nair Sivakumari, Sankar Jagadeeshan, Jisha J. Pillai, Ashalatha Akhilandeshwaran, Gopalakrishnapillai Sankaramangalam Vinod Kumar |
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| Rok vydání: | 2012 |
| Předmět: |
Drug
Drug Compounding Cellular differentiation media_common.quotation_subject Retinoic acid Antineoplastic Agents HL-60 Cells Tretinoin Pharmacology chemistry.chemical_compound Microscopy Electron Transmission Drug Discovery Humans Particle Size Solubility Polyglactin 910 media_common Drug Carriers Chemistry Cell Differentiation Controlled release In vitro Bioavailability Kinetics Leukemia Myeloid Acute Apoptosis Nanoparticles Emulsions Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Medicinal Chemistry. 8:805-810 |
| ISSN: | 1573-4064 |
| DOI: | 10.2174/157340612802084333 |
| Popis: | All-trans-retinoic acid reverses malignant cell growth and induces cell differentiation and apoptosis. Poor aqueous solubility and uncertain bioavailability are the limiting factors for using all-trans-retinoic acid for tumor therapy. The objective of present study was to encapsulate the hydrophobic drug all-trans-retinoic acid in the polymer poly (lactide-coglycolide). The encapsulation was expected to improve the bioavailability and solubility of the drug. Oil in water single emulsion solvent evaporation technique used for the preparation efficiently encapsulated about 60% of the drug. The drug release profile showed a biphasic pattern with 70% of the drug being released in first 48 hrs and the residual drug showing a slow controlled release reaching up to 8 days. The particle size of 150-200 nm as determined with TEM was ideal for tumor targeting. All-trans-retinoic acid loaded nanoparticles were efficient to induce differentiation and blocked the proliferation of HL-60 cells invitro. These studies also revealed that the dosage of drug required for the therapeutic effects have been reduced efficiently. Our studies thereby demonstrate that Poly (lactide-co-glycolide) based nanoparticles may be efficient for parenteral administration of the drug. |
| Databáze: | OpenAIRE |
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