Components of the Canonical and Non-Canonical Wnt Pathways Are Not Mis-Expressed in Pituitary Tumors
| Autor: | Renata Costa Camargo, Leandro M. Colli, Sonir Roberto Rauber Antonini, Luciano Neder Serafini, Fabiano Pinto Saggioro, Hélio Rubens Machado, Margaret de Castro, Ayrton Custódio Moreira |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Pituitary gland
IMUNOHISTOQUÍMICA Science Pituitary neoplasm Biology Pituitary Tumors Endocrinology Molecular Cell Biology medicine Signaling in Cellular Processes Cluster Analysis Humans Pituitaryadenomas Pituitary Neoplasms Endocrine Tumors Neurological Tumors Wnt Signaling Pathway WNT Signaling Cascade Regulation of gene expression Multidisciplinary Endocrine Physiology Pituitary tumors Wnt signaling pathway LRP6 Cancers and Neoplasms LRP5 Beta-Catenin Signaling medicine.disease Immunohistochemistry Signaling Cascades Neoplasm Proteins Gene Expression Regulation Neoplastic medicine.anatomical_structure Pituitary Oncology Cancer research Medicine PTK7 Research Article Signal Transduction |
| Zdroj: | PLoS ONE Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP PLoS ONE, Vol 8, Iss 4, p e62424 (2013) |
| ISSN: | 1932-6203 |
| Popis: | IntroductionCanonical and non-canonical Wnt pathways are involved in the genesis of multiple tumors; however, their role in pituitary tumorigenesis is mostly unknown.ObjectiveThis study evaluated gene and protein expression of Wnt pathways in pituitary tumors and whether these expression correlate to clinical outcome.Materials and methodsGenes of the WNT canonical pathway: activating ligands (WNT11, WNT4, WNT5A), binding inhibitors (DKK3, sFRP1), β-catenin (CTNNB1), β-catenin degradation complex (APC, AXIN1, GSK3β), inhibitor of β-catenin degradation complex (AKT1), sequester of β-catenin (CDH1), pathway effectors (TCF7, MAPK8, NFAT5), pathway mediators (DVL-1, DVL-2, DVL-3, PRICKLE, VANGL1), target genes (MYB, MYC, WISP2, SPRY1, TP53, CCND1); calcium dependent pathway (PLCB1, CAMK2A, PRKCA, CHP); and planar cell polarity pathway (PTK7, DAAM1, RHOA) were evaluated by QPCR, in 19 GH-, 18 ACTH-secreting, 21 non-secreting (NS) pituitary tumors, and 5 normal pituitaries. Also, the main effectors of canonical (β-catenin), planar cell polarity (JNK), and calcium dependent (NFAT5) Wnt pathways were evaluated by immunohistochemistry.ResultsThere are no differences in gene expression of canonical and non-canonical Wnt pathways between all studied subtypes of pituitary tumors and normal pituitaries, except for WISP2, which was over-expressed in ACTH-secreting tumors compared to normal pituitaries (4.8x; p = 0.02), NS pituitary tumors (7.7x; p = 0.004) and GH-secreting tumors (5.0x; p = 0.05). β-catenin, NFAT5 and JNK proteins showed no expression in normal pituitaries and in any of the pituitary tumor subtypes. Furthermore, no association of the studied gene or protein expression was observed with tumor size, recurrence, and progressive disease. The hierarchical clustering showed a regular pattern of genes of the canonical and non-canonical Wnt pathways randomly distributed throughout the dendrogram.ConclusionsOur data reinforce previous reports suggesting no activation of canonical Wnt pathway in pituitary tumorigenesis. Moreover, we describe, for the first time, evidence that non-canonical Wnt pathways are also not mis-expressed in the pituitary tumors. |
| Databáze: | OpenAIRE |
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