Mapping O2 concentration in ex-vivo tissue samples on a fast PLIM macro-imager
Autor: | Rajannya Sen, Liisa M. Hirvonen, Patrick Fitzgerald, Thomaz F.S. Bastiaanssen, John F. Cryan, Alexander V. Zhdanov, Peter Svihra, Stefan Andersson-Engels, Dmitri B. Papkovsky, Andrei Nomerotski |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Cell Respiration lcsh:Medicine Tissue surface Article Imaging Mice 03 medical and health sciences Medical research 0302 clinical medicine Microscopy medicine Animals Humans Image acquisition Tissue Distribution Large intestine lcsh:Science Sensors and probes Colonic lumen Biological models Multidisciplinary Tissue respiration Chemistry Optical Imaging lcsh:R HCT116 Cells Oxygen HEK293 Cells 030104 developmental biology medicine.anatomical_structure Optics and photonics Molecular Probes Nanoparticles Female lcsh:Q 030217 neurology & neurosurgery Ex vivo Biomedical engineering Lumen (unit) |
Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020) Scientific Reports |
ISSN: | 2045-2322 |
DOI: | 10.1038/s41598-020-75928-3 |
Popis: | O2 PLIM microscopy was employed in various studies, however current platforms have limitations in sensitivity, image acquisition speed, accuracy and general usability. We describe a new PLIM imager based on the Timepix3 camera (Tpx3cam) and its application for imaging of O2 concentration in various tissue samples stained with a nanoparticle based probe, NanO2-IR. Upon passive staining of mouse brain, lung or intestinal tissue surface with minute quantities of NanO2-IR or by microinjecting the probe into the lumen of small or large intestine fragments, robust phosphorescence intensity and lifetime signals were produced, which allow mapping of O2 in the tissue within 20 s. Inhibition of tissue respiration or limitation of O2 diffusion to tissue produced the anticipated increases or decreases in O2 levels, respectively. The difference in O2 concentration between the colonic lumen and air-exposed serosal surface was around 140 µM. Furthermore, subcutaneous injection of 5 µg of the probe in intact organs (a paw or tail of sacrificed mice) enabled efficient O2 imaging at tissue depths of up to 0.5 mm. Overall, the PLIM imager holds promise for metabolic imaging studies with various ex vivo models of animal tissue, and also for use in live animals. |
Databáze: | OpenAIRE |
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