Molecular characterization of a trafficking organelle: dissecting the axonal paths of calsyntenin-1 transport vesicles
| Autor: | Alexander Stephan, Beat Kunz, Peter Sonderegger, Tu-My Diep, Alexander Ludwig, Bertran Gerrits, Martin Steuble, José María Mateos, Peter Streit, Mitsuo Tagaya, Philipp Schätzle |
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| Přispěvatelé: | University of Zurich, Sonderegger, P |
| Rok vydání: | 2010 |
| Předmět: |
Proteomics
Nervous system 1303 Biochemistry Endosome Kinesins 610 Medicine & health 10071 Functional Genomics Center Zurich Endosomes Biology Hippocampus Biochemistry Amyloid beta-Protein Precursor Mice 03 medical and health sciences Prosencephalon 0302 clinical medicine Organelle 10019 Department of Biochemistry 1312 Molecular Biology medicine Amyloid precursor protein Animals Molecular Biology 030304 developmental biology 0303 health sciences Vesicle Calcium-Binding Proteins Colocalization Biological Transport Immunohistochemistry Axons Endocytosis Cell biology medicine.anatomical_structure nervous system biology.protein Axoplasmic transport 570 Life sciences biology Kinesin Electrophoresis Polyacrylamide Gel 030217 neurology & neurosurgery |
| Zdroj: | Proteomics |
| DOI: | 10.5167/uzh-40014 |
| Popis: | Kinesin motors play crucial roles in the delivery of membranous cargo to its destination and thus for the establishment and maintenance of cellular polarization. Recently, calsyntenin-1 was identified as a cargo-docking protein for Kinesin-1-mediated axonal transport of tubulovesicular organelles along axons of central nervous system neurons. To further define the function of calsyntenin-1, we immunoisolated calsyntenin-1 organelles from murine brain homogenates and determined their proteome by MS. We found that calsyntenin-1 organelles are endowed with components of the endosomal trafficking machinery and contained the β-amyloid precursor protein (APP). Detailed biochemical analyses of calsyntenin-1 immunoisolates in conjunction with immunocytochemical colocalization studies with cultured hippocampal neurons, using endosomal marker proteins for distinct subcompartments of the endosomal pathways, indicated that neuronal axons contain at least two distinct, nonoverlapping calsyntenin-1-containing transport packages: one characterized as early-endosomal, APP positive, the other as recycling-endosomal, APP negative. We postulate that calsyntenin-1 acts as a general mediator of anterograde axonal transportation of endosomal vesicles. In this role, calsyntenin-1 may actively contribute to axonal growth and pathfinding in the developing as well as to the maintenance of neuronal polarity in the adult nervous system; further, it may actively contribute to the stabilization of APP during its anterograde axonal trajectory. |
| Databáze: | OpenAIRE |
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