Acyl coenzyme A:Retinol acyltransferase activity and the vitamin A content of polar bear (Ursus maritimus) liver
| Autor: | Harold C. Furr, James Allen Olson, Mark D. Ball |
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| Rok vydání: | 1986 |
| Předmět: |
Male
Vitamin Physiology Carnivora Biochemistry Dithiothreitol chemistry.chemical_compound Retinyl palmitate Animals Bovine serum albumin Vitamin A Molecular Biology biology Retinol O-Fatty-Acyltransferase Retinol General Medicine Metabolism biology.organism_classification Kinetics Liver chemistry Microsoma Microsomes Liver Microsome biology.protein Animals Zoo Acyltransferases Ursidae |
| Zdroj: | Comparative Biochemistry and Physiology Part B: Comparative Biochemistry. 84:513-517 |
| ISSN: | 0305-0491 |
| Popis: | 1. 1. Acyl coenzyme A: retinol acyltransferase activity was identified in the microsomes from a polar bear liver. 2. 2. The highest rate of in vitro retinol esterification was 821 pmol/min/mg microsomal protein. 3. 3. The in vitro esterification rate displayed a small dependence upon the concentration of exogenous protein (bovine serum albumin) and even less on the concentration of sulfhydryl-reducing agent (dithiothreitol). 4. 4. Vitamin A was present in the liver at a concentration of 8050 μg/g tissue, with 98% of the vitamin in its ester form. 5. 5. Retinyl palmitate was 37.3% of the total liver retinyl esters, while retinyl oleate represented 20.9%, stearate 12.8%, and linoleate 7.7%. |
| Databáze: | OpenAIRE |
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