A lactose-binding lectin from the marine sponge Cinachyrella apion (Cal) induces cell death in human cervical adenocarcinoma cells
| Autor: | Adriana F. Uchoa, Paula Ivani Medeiros dos Santos, Norberto K.V. Monteiro, Luciana Maria Araújo Rabêlo, Elizeu Antunes dos Santos, Adeliana S. Oliveira, Ana Heloneida de Araújo Morais, Raphael Paschoal Serquiz, Hugo Alexandre Oliveira Rocha, Ruth Medeiros Oliveira |
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| Rok vydání: | 2012 |
| Předmět: |
Programmed cell death
Pharmaceutical Science Uterine Cervical Neoplasms Apoptosis Lactose Adenocarcinoma Article Flow cytometry S Phase HeLa Mice Necrosis Cell Line Tumor Lectins Drug Discovery medicine Cytotoxic T cell Animals Humans Cinachyrella apion Pharmacology Toxicology and Pharmaceutics (miscellaneous) lcsh:QH301-705.5 Cell Proliferation bcl-2-Associated X Protein antitumor biology medicine.diagnostic_test Cell Death Cell growth Hemolytic Agents lectin marine sponge Lactose binding 3T3 Cells Cell Cycle Checkpoints biology.organism_classification Molecular biology Cell biology Mitochondria Porifera lcsh:Biology (General) Cell culture Caspases Female Lectin HeLa Cells |
| Zdroj: | Marine Drugs Marine Drugs, Vol 10, Iss 4, Pp 727-743 (2012) Marine Drugs; Volume 10; Issue 4; Pages: 727-743 |
| ISSN: | 1660-3397 |
| Popis: | Cancer represents a set of more than 100 diseases, including malignant tumors from different locations. Strategies inducing differentiation have had limited success in the treatment of established cancers. Marine sponges are a biological reservoir of bioactive molecules, especially lectins. Several animal and plant lectins were purified with antitumor activity, mitogenic, anti-inflammatory and antiviral, but there are few reports in the literature describing the mechanism of action of lectins purified from marine sponges to induce apoptosis in human tumor cells. In this work, a lectin purified from the marine sponge Cinachyrella apion (CaL) was evaluated with respect to its hemolytic, cytotoxic and antiproliferative properties, besides the ability to induce cell death in tumor cells. The antiproliferative activity of CaL was tested against HeLa, PC3 and 3T3 cell lines, with highest growth inhibition for HeLa, reducing cell growth at a dose dependent manner (0.5-10 µg/mL). Hemolytic activity and toxicity against peripheral blood cells were tested using the concentration of IC(50) (10 µg/mL) for both trials and twice the IC(50) for analysis in flow cytometry, indicating that CaL is not toxic to these cells. To assess the mechanism of cell death caused by CaL in HeLa cells, we performed flow cytometry and western blotting. Results showed that lectin probably induces cell death by apoptosis activation by pro-apoptotic protein Bax, promoting mitochondrial membrane permeabilization, cell cycle arrest in S phase and acting as both dependent and/or independent of caspases pathway. These results indicate the potential of CaL in studies of medicine for treating cancer. |
| Databáze: | OpenAIRE |
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