Yielding quality viral RNA by using two different chemistries: a comparative performance study
| Autor: | Srikanta Kanungo, Jyotsnamayee Sabat, Saroj Dash, Lal Mohan Ho, Sailendra Panda, Subhra Subhadra, Jyotirmayee Turuk, Sanghamitra Pati, Madhab Charan Mandal, Sonalika Rath |
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| Rok vydání: | 2021 |
| Předmět: |
2019-20 coronavirus outbreak
SARS-CoV-2 Gene Expression Profiling Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) COVID-19 RNA Membranes Artificial Computational biology Biology Silicon Dioxide Polymerase Chain Reaction General Biochemistry Genetics and Molecular Biology COVID-19 Nucleic Acid Testing Magnetic bead Gene expression Magnets Nucleic acid Humans RNA Viral Viral rna Reagent Kits Diagnostic RNA extraction Biotechnology |
| Zdroj: | BioTechniques. 71:510-515 |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/btn-2021-0054 |
| Popis: | Purity and integrity are two important criteria for any RNA extraction process to qualify the RNA for meaningful gene expression analysis. This study compares four commercially available RNA extraction kits using silica membrane and magnetic bead separation methods. The performance was evaluated in terms of both quantity (total RNA amount in μg/μl) and purity (260/280 ratio). The concentration and purity of each kit was significantly different from those of the others (p |
| Databáze: | OpenAIRE |
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