Protein-protein interactions of the nicotinamide/nicotinate mononucleotide adenylyltransferase of Leishmania braziliensis
| Autor: | María Helena Ramírez-Hernández, Luis Ernesto Contreras-Rodríguez, Lesly Johanna Ortiz-Joya |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Models
Molecular Microbiology (medical) lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 030231 tropical medicine Co-IP-MS/MS lcsh:QR1-502 Nicotinamide adenine dinucleotide Leishmania braziliensis lcsh:Microbiology Protein–protein interaction protein-protein interaction 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Protein Interaction Mapping Gene expression Nicotinamide-Nucleotide Adenylyltransferase chemistry.chemical_classification biology Nicotinamide NMNAT biology.organism_classification NAD Enzyme chemistry Biochemistry Original Article Protein folding NAD+ kinase Signal Transduction |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 114, Iss 0 (2019) Memórias do Instituto Oswaldo Cruz, Volume: 114, Article number: e180506, Published: 21 MAR 2019 Memórias do Instituto Oswaldo Cruz |
| ISSN: | 1678-8060 |
| Popis: | Background Nicotinamide adenine dinucleotide (NAD) plays a central role in energy metabolism and integrates cellular metabolism with signalling and gene expression. NAD biosynthesis depends on the enzyme nicotinamide/nicotinate mononucleotide adenylyltransferase (NMNAT; EC: 2.7.7.1/18), in which converge the de novo and salvage pathways. Objective The purpose of this study was to analyse the protein-protein interactions (PPI) of NMNAT of Leishmania braziliensis (LbNMNAT) in promastigotes. Methods Transgenic lines of L. braziliensis promastigotes were established by transfection with the pSP72αneoαLbNMNAT-GFP vector. Soluble protein extracts were prepared, co-immunoprecipitation assays were performed, and the co-immunoprecipitates were analysed by mass spectrometry. Furthermore, bioinformatics tools such as network analysis were applied to generate a PPI network. Findings Proteins involved in protein folding, redox homeostasis, and translation were found to interact with the LbNMNAT protein. The PPI network indicated enzymes of the nicotinate and nicotinamide metabolic routes, as well as RNA-binding proteins, the latter being the point of convergence between our experimental and computational results. Main conclusion We constructed a model of PPI of LbNMNAT and showed its association with proteins involved in various functions such as protein folding, redox homeostasis, translation, and NAD synthesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|