Role of Mycobacterium paratuberculosis in Crohn's disease
| Autor: | Michael E. Presti, Paul E. Zachary, W. X. Fan, Paul F. Petersen, Walter E. Longo, Wendell K. Clarkston, Craig L. Leonardi, John M. Kreeger, Anthony M. Vernava |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent Colon Paratuberculosis Disease Polymerase Chain Reaction law.invention Pathogenesis Crohn Disease Intestinal mucosa law Biopsy medicine Humans Prospective Studies Intestinal Mucosa Polymerase chain reaction Aged Aged 80 and over Crohn's disease medicine.diagnostic_test business.industry Gastroenterology General Medicine Middle Aged medicine.disease Ulcerative colitis digestive system diseases Mycobacterium avium subsp. paratuberculosis Colitis Ulcerative Female business |
| Zdroj: | Diseases of the Colon & Rectum. 41:195-199 |
| ISSN: | 0012-3706 |
| DOI: | 10.1007/bf02238248 |
| Popis: | PURPOSE:Mycobacterium paratuberculosis has been proposed as a causative agent in patients with Crohn's disease. The purpose of this study was to determine whetherM. paratuberculosis was present in tissue from patients with Crohn's disease in a defined geographic area. METHODS: We prospectively evaluated, using polymerase chain reaction and culture, whetherM. paratuberculosis was present in 44 specimens (37 from intestinal mucosal biopsies and 7 from surgical resections) from patients with Crohn's disease, ulcerative colitis, or normal colonic mucosa. RESULTS: Of the 25 specimens tested from the 21 Crohn's patients, only 1 positive specimen was noted, whereas the 8 specimens from the 5 ulcerative colitis patients and the 11 specimens from the 11 control patients failed to demonstrate a positive result with polymerase chain reaction. Cultures of all specimens revealed no growth ofM. paratuberculosis. CONCLUSION:M. paratuberculosis was only rarely detected in biopsy or surgical specimens from patients with Crohn's disease. These results do not support a common causative role ofM. paratuberculosis in Crohn's disease. |
| Databáze: | OpenAIRE |
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