How to perform a nanoindentation experiment on a virus
| Autor: | Roos, W.H., Peterman, EJG, Wuite, GJL |
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| Přispěvatelé: | Physics of Living Systems, LaserLaB - Molecular Biophysics, Peterman, EJG, Wuite, GJL, Molecular Biophysics |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
atomic force microscopy
nanotechnology Atomic force microscopy Force spectroscopy Biophysics article Modulus Mechanical properties methodology virus Nanoindentation chemistry Materials science virus capsid Capsid bacteriophage Surface preparation Viral structure Virus Structure Deformation (engineering) Composite material Material properties |
| Zdroj: | Single Molecule Analysis, Series Methods in Molecular Biology Roos, W H 2011, How to perform a nanoindentation experiment on a virus . in EJG Peterman & GJL Wuite (eds), Single Molecule Analysis, Series Methods in Molecular Biology . Humama press, New York . https://doi.org/10.1007/978-1-61779-282-3_14 Single Molecule Analysis ISBN: 9781617792816 Methods in Molecular Biology: Single Molecule Analysis, 783, 251-264 |
| Popis: | To broaden our knowledge on virus structure and function, a profound insight into their mechanical properties is required. Nanoindentation measurements with an atomic force microscope (AFM) are increasingly being performed to probe such material properties. This single-particle approach allows for determining the viral spring constant, their Young's modulus, as well as the force and deformation at which failure occurs. The experimental procedures for viral nanoindentation experiments are described here in detail, focusing on surface preparation, AFM imaging and nanoindentation, and the subsequent data analysis of the force-distance curves. Whereas AFM can be operated in air and in liquid, the described methods are for probing single viruses in liquid to enable working in a physiologically relevant environment. © Springer Science+Business Media, LLC 2011. |
| Databáze: | OpenAIRE |
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