Monitoring Tumor Glucose Utilization by Positron Emission Tomography for the Prediction of Treatment Response to Epidermal Growth Factor Receptor Kinase Inhibitors
| Autor: | Harvey R. Herschman, Wolfgang Weber, Yann Seimbille, Claudia Bodenstein, Rebecca A. Dumont, Steven M. Dubinett, Helen Su, Johannes Czernin, Michael E. Phelps |
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| Rok vydání: | 2006 |
| Předmět: |
Cancer Research
medicine.medical_specialty Lung Neoplasms Antineoplastic Agents Mice SCID Biology Sensitivity and Specificity Mice Gefitinib Growth factor receptor Fluorodeoxyglucose F18 Carcinoma Non-Small-Cell Lung Internal medicine Tumor Cells Cultured medicine Animals Humans Epidermal growth factor receptor Neoplasm Staging EGFR inhibitors Fluorodeoxyglucose Kinase Glucose transporter ErbB Receptors Glucose Treatment Outcome Endocrinology Oncology Positron-Emission Tomography Quinazolines biology.protein Cancer research medicine.drug GLUT3 |
| Zdroj: | Clinical Cancer Research. 12:5659-5667 |
| ISSN: | 1557-3265 1078-0432 |
| DOI: | 10.1158/1078-0432.ccr-06-0368 |
| Popis: | Purpose: The mechanisms underlying the sensitivity of non–small cell lung cancer to epidermal growth factor receptor (EGFR) kinase inhibitors are complex, and there are no established markers to accurately predict treatment outcome in individual patients.Experimental Design: We investigated whether tumors responding to EGFR inhibitors can be identified by measuring treatment-induced changes in glucose utilization by positron emission tomography with the glucose analogue fluorodeoxyglucose (FDG-PET). We studied a panel of cell lines with a spectrum of sensitivity to EGFR kinase inhibitors. After incubation with the EGFR kinase inhibitor gefitinib for various time points, FDG uptake, glucose transport rates, and hexokinase activity were determined. FDG uptake in vivo was assessed by microPET imaging of tumor xenografts in mice.Results: In gefitinib-sensitive cell lines, there was a dramatic decrease in FDG uptake as early as 2 hours after treatment. Immunoblots showed the translocation of glucose transporters (GLUT3) from the plasma membrane to the cytosol; glucose transport rates were reduced 2.6-fold at this time. There was also a modest reduction of hexokinase activity. These metabolic alterations preceded changes in cell cycle distribution, thymidine uptake, and apoptosis. MicroPET studies showed an up to 55% decrease of tumor FDG uptake in sensitive xenografts within 48 hours. In contrast, gefitinib-resistant cells exhibited no measurable changes in FDG uptake, either in cell culture or in vivo.Conclusion: Glucose metabolic activity closely reflects response to gefitinib therapy. FDG-PET may be a valuable clinical predictor, early in the course of treatment, for therapeutic responses to EGFR kinase inhibitors. |
| Databáze: | OpenAIRE |
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