Establishment of an immunological detection method of fleroxacin by fluorescence-linked immunosorbent assay

Autor:	Yajie Wang, Aiping Wang, Yumei Chen, Liu Yankai, Hongliang Liu, Yanhua Qi, Jingming Zhou, Luhui Zhao, Yuanyuan Zhang
Rok vydání:	2021
Předmět:	Fleroxacin medicine.drug_class Health Toxicology and Mutagenesis Enzyme-Linked Immunosorbent Assay 02 engineering and technology Toxicology Monoclonal antibody 01 natural sciences Fluorescence Mice Quantum Dots medicine Animals Mice Inbred BALB C Chromatography Chemistry 010401 analytical chemistry technology industry and agriculture Public Health Environmental and Occupational Health Antibodies Monoclonal Serum Albumin Bovine General Chemistry General Medicine equipment and supplies 021001 nanoscience & nanotechnology 0104 chemical sciences Ammonium Sulfate Quantum dot Caprylates 0210 nano-technology Food Science medicine.drug
Zdroj:	Food Additives & Contaminants: Part A. 38:594-601
ISSN:	1944-0057 1944-0049
DOI:	10.1080/19440049.2021.1876251
Popis:	The direct and indirect competitive fluorescence-linked immunosorbent assay (FLISA and icFLISA) incorporating quantum dots (QDs) for the detection of fleroxacin (FLE) was established for the first time in this study. The monoclonal antibody specific for FLE was successfully conjugated with QDs after purification by the caprylic acid-ammonium sulphate method. The limits of detection of FLISA and icFLISA were 0.012 ng/mL and 0.006 ng/mL, respectively; IC
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4f97f53170aa69a05998742a0961cad https://doi.org/10.1080/19440049.2021.1876251 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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