DNA barcoding of British mosquitoes (Diptera, Culicidae) to support species identification, discovery of cryptic genetic diversity and monitoring invasive species
| Autor: | Nicholas Johnson, Nadya Nikolova, Leigh Thorne, Victor A. Brugman, Mar Fernández de Marco, Andreas Krüger, Ignacio Ruiz-Arrondo, Elsa Barrero, Sarah Lumley, Luis M. Hernández-Triana, Anthony R. Fooks |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Insecta Arthropoda Culex 030231 tropical medicine Zoology molecular identification DNA barcoding 03 medical and health sciences 0302 clinical medicine lcsh:Zoology parasitic diseases Genetics Animalia lcsh:QL1-991 Culiseta Ecology Evolution Behavior and Systematics Aedes Genetic diversity biology Diptera Anopheles biology.organism_classification 3. Good health Genetic divergence Europe 030104 developmental biology Culicidae Genetic marker Animal Science and Zoology vector species DNA extraction methods Research Article hidden genetic diversity |
| Zdroj: | ZooKeys ZooKeys 832: 57-76 ZooKeys, Vol 832, Iss, Pp 57-76 (2019) |
| ISSN: | 1313-2970 1313-2989 |
| Popis: | Correct mosquito species identification is essential for mosquito and disease control programs. However, this is complicated by the difficulties in morphologically identifying some mosquito species. In this study, variation of a partial sequence of the cytochromecoxidase unit I (COI) gene was used for the molecular identification of British mosquito species and to facilitate the discovery of cryptic diversity, and monitoring invasive species. Three DNA extraction methods were compared to obtain DNA barcodes from adult specimens. In total, we analyzed 42 species belonging to the generaAedesMeigen, 1818 (21 species),AnophelesMeigen, 1818 (7 species),CoquillettidiaTheobald, 1904 (1 species),CulexLinnaeus, 1758 (6 species),CulisetaFelt, 1904 (7 species), andOrthopodomyiaTheobald, 1904 (1 species). Intraspecific genetic divergence ranged from 0% to 5.4%, while higher interspecific divergences were identified betweenAedesgeminusPeus, 1971/Culisetalitorea(Shute, 1928) (24.6%) andAe.geminus/An.plumbeusStephens, 1828 (22.5%). Taxonomic discrepancy was shown betweenAn.daciaeLinton, Nicolescu & Harbach, 2004 andAn.messeaeFalleroni, 1828 indicating the poor resolution of theCOIDNA barcoding region in separating these taxa. Other species such asAe.cantans(Meigen, 1818)/Ae.annulipes(Meigen, 1830) showed similar discrepancies indicating some limitation of this genetic marker to identify certain mosquito species. The combination of morphology and DNA barcoding is an effective approach for the identification of British mosquitoes, for invasive mosquitoes posing a threat to the UK, and for the detection of hidden diversity within species groups. |
| Databáze: | OpenAIRE |
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