The Pulmonary Hypertensive Fawn-hooded Rat Has a Normal Serotonin Transporter Coding Sequence
| Autor: | Ana-Maria Gonzalez, Celia Emery, Timothy W Higenbottam, Adrian P. L. Smith |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Blood Platelets
Male Platelet Storage Pool Deficiency Pulmonary and Respiratory Medicine Serotonin medicine.medical_specialty DNA Complementary Serotonin uptake Brown rat Hypertension Pulmonary Molecular Sequence Data Clinical Biochemistry Nerve Tissue Proteins Biology Species Specificity Internal medicine medicine Animals Humans Platelet Amino Acid Sequence Rats Wistar Molecular Biology Peptide sequence Serotonin transporter DNA Primers Serotonin Plasma Membrane Transport Proteins Membrane Glycoproteins Base Sequence Sequence Homology Amino Acid Membrane Transport Proteins Cell Biology medicine.disease biology.organism_classification Pulmonary hypertension Rats Endocrinology embryonic structures biology.protein Carrier Proteins |
| Zdroj: | American Journal of Respiratory Cell and Molecular Biology. 19:245-249 |
| ISSN: | 1535-4989 1044-1549 |
| DOI: | 10.1165/ajrcmb.19.2.3073 |
| Popis: | The coding sequence of the serotonin transporter gene was compared in two strains of rat-the Wistar and the fawn-hooded rat (FHR). The FHR has an inherited platelet storage-pool deficiency and a widespread impairment of serotonin storage. It is also susceptible to systemic and pulmonary hypertension. The FHR provides a model to study the genetics in human systemic and pulmonary hypertension. We measured platelet function in these two strains by measuring incorporation of radiolabeled serotonin into a platelet suspension and found significant differences in serotonin uptake and release. The coding sequence for the serotonin transporter in the FHR has yet to be reported. No differences were found in the predicted amino acid sequence between these two strains of rat, either in the platelet or the lung samples or when compared with the published sequence of the brown rat. We conclude that differences in the primary structure of the serotonin transporter gene do not account for the altered serotonin storage in the FHR strain. |
| Databáze: | OpenAIRE |
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