Azoreductase from alkaliphilic Bacillus sp. AO1 catalyzes indigo reduction
| Autor: | Toshihisa Ohshima, Hirokazu Suzuki, Tomoaki Abe, Katsumi Doi |
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| Rok vydání: | 2018 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Dinitrocresols 030106 microbiology Bacillus Flavoprotein Reductase Indigo Carmine Applied Microbiology and Biotechnology Catalysis Indigo 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Alkaliphile NADH NADPH Oxidoreductases Amino Acid Sequence Coloring Agents Phylogeny chemistry.chemical_classification Base Sequence Flavoproteins biology Sequence Analysis DNA General Medicine Nitroreductases NAD biology.organism_classification Bacterial Typing Techniques 030104 developmental biology Enzyme chemistry Biochemistry Indigo carmine biology.protein ATP-Binding Cassette Transporters Polygonum Dyeing Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 102:9171-9181 |
| ISSN: | 1432-0614 0175-7598 |
| Popis: | Indigo is an insoluble blue dye historically used for dyeing textiles. A traditional approach for indigo dyeing involves microbial reduction of polygonum indigo to solubilize it under alkaline conditions; however, the mechanism by which microorganisms reduce indigo remains poorly understood. Here, we aimed to identify an enzyme that catalyzes indigo reduction; for this purpose, from alkaline liquor that performed microbial reduction of polygonum indigo, we isolated indigo carmine-reducing microorganisms. All isolates were facultative anaerobic and alkali-tolerant Bacillus spp. An isolate termed AO1 was found to be an alkaliphile that preferentially grows at pH 9.0–11.0 and at 30–35 °C. We focused on flavin-dependent azoreductase as a possible enzyme for indigo carmine reduction and identified its gene (azoA) in Bacillus sp. AO1 using homology-based strategies. azoA was monocistronic but clustered with ABC transporter genes. Primary sequence identities were |
| Databáze: | OpenAIRE |
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