Fermentation of crude glycerol from biodiesel production by Clostridium pasteurianum
| Autor: | Marie Just Mikkelsen, Thomas Kvist, Peter Vittrup Christensen, Torbjørn Ølshøj Jensen, Peter Westermann |
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| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
Glycerol Butanols chemistry.chemical_element Bioengineering 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound Clostridium 010608 biotechnology Food science 030304 developmental biology 0303 health sciences Biodiesel biology Butanol biology.organism_classification Carbon chemistry Biochemistry Biofuel Propylene Glycols Biodiesel production Biofuels Fermentation Biotechnology |
| Zdroj: | Journal of Industrial Microbiology & Biotechnology |
| ISSN: | 1476-5535 |
| Popis: | Clostridium pasteurianum can utilize glycerol as the sole carbon source for the production of butanol and 1,3-propanediol. Crude glycerol derived from biodiesel production has been shown to be toxic to the organism even in low concentrations. By examination of different pretreatments we found that storage combined with activated stone carbon addition facilitated the utilization of crude glycerol. A pH-controlled reactor with in situ removal of butanol by gas stripping was used to evaluate the performance. The fermentation pattern on pretreated crude glycerol was quite similar to that on technical grade glycerol. C. pasteurianum was able to utilize 111 g/l crude glycerol. The average consumption rate was 2.49 g/l/h and maximum consumption rate was 4.08 g/l/h. At the maximal glycerol consumption rate butanol was produced at 1.3 g/l/h. These rates are higher than those previously reported for fermentations on technical grade glycerol by the same strain. A process including pretreatment and subsequent fermentation of the crude glycerol could be usable for industrial production of butanol by C. pasteurianum. |
| Databáze: | OpenAIRE |
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