Lentivirus transduction of bone marrow hemopoietic precursor cells with Lin-c-kit+ phenotype Ex Vivo using a genetic construct containing green fluorescent protein gene
| Autor: | T. N. Vlasik, N. V. Radyukhina, I. N. Rybalkin, T. I. Aref’yeva, P.N. Rutkevich, M. A. Slinkin, T. Kh. Gurskaya, A. Ya. Shevelyov, O.P. Ilyinskaya, Eduard Tararak |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Transgene Genetic Vectors Green Fluorescent Proteins Lentivirus Hematopoietic Stem Cell Transplantation General Medicine Biology Hematopoietic Stem Cells Molecular biology General Biochemistry Genetics and Molecular Biology Green fluorescent protein Mice Transduction (genetics) Haematopoiesis medicine.anatomical_structure Transduction Genetic In vivo Precursor cell medicine Animals Female Bone marrow Ex vivo |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 143:723-726 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-007-0224-6 |
| Popis: | We developed a technology of labeling bone marrow precursor cells with the Lin-c-kit+ phenotype in culture by green fluorescent protein gene using a lentivirus vector. The proposed system provides effective transduction of bone marrow precursor cells and high transgene expression level in vitro (27%). The integration of the transgene into the transduced cell genome in vivo was verified by the method of splenic colonies. |
| Databáze: | OpenAIRE |
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