Characterization of the Transcriptome and Gene Expression of Tetraploid Black Locust Cuttings in Response to Etiolation
| Autor: | Zijing Luo, Hongjing Duan, Yun Li, Dai Li, Yuhan Sun, Shaoming Wang, Nan Lu, Yanzhong Wen, Rongxuan Hou |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine lcsh:QH426-470 phytohormone 01 natural sciences Article Transcriptome 03 medical and health sciences Botany Gene expression Genetics etiolation Hormone metabolism Gene Genetics (clinical) Oxidase test biology peroxidase activity food and beverages biology.organism_classification Cell biology lcsh:Genetics 030104 developmental biology Tetraploid Robinia pseudoacacia L transcriptome Etiolation biology.protein Locust 010606 plant biology & botany Peroxidase |
| Zdroj: | Genes; Volume 8; Issue 12; Pages: 345 Genes Genes, Vol 8, Iss 12, p 345 (2017) |
| ISSN: | 2073-4425 |
| Popis: | Etiolation (a process of growing plants in partial or complete absence of light) promotes adventitious root formation in tetraploid black locust (Robinia pseudoacacia L.) cuttings. We investigated the mechanism underlying how etiolation treatment promotes adventitious root formation in tetraploid black locust and assessed global transcriptional changes after etiolation treatment. Solexa paired-end sequencing of complementary DNAs (cDNAs) from control (non-etiolated, NE) and etiolated (E) samples resulted in 107,564 unigenes. In total, 52,590 transcripts were annotated and 474 transcripts (211 upregulated and 263 downregulated) potentially involved in etiolation were differentially regulated. These genes were associated with hormone metabolism and response, photosynthesis, signaling pathways, and starch and sucrose metabolism. In addition, we also found significant differences of phytohormone contents, activity of following enzymes i.e., peroxidase, polyphenol oxidase and indole acetic acid oxidase between NE and E tissues during some cottage periods. The genes responsive to etiolation stimulus identified in this study will provide the base for further understanding how etiolation triggers adventitious roots formation in tetraploid black locus. |
| Databáze: | OpenAIRE |
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