Functional analysis of interactions between Tat and the trans-activation response element of human immunodeficiency virus type 1 in cells
| Autor: | Tristram G. Parslow, S. J. Madore, B M Peterlin, Ying Luo, Bryan R. Cullen |
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| Rok vydání: | 1993 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Transcriptional Activation Transcription Genetic viruses Molecular Sequence Data Immunology Gene Expression RNA-binding protein Biology Transfection Microbiology Cell Line Transcription (biology) Virology Gene expression Animals RNA Messenger HIV Long Terminal Repeat Messenger RNA Base Sequence RNA rev Gene Products Human Immunodeficiency Virus Molecular biology Recombinant Proteins Long terminal repeat Gene Products rev Regulatory sequence Insect Science Gene Products tat HIV-1 Nucleic Acid Conformation RNA Viral tat Gene Products Human Immunodeficiency Virus Plasmids Protein Binding Research Article |
| Zdroj: | Journal of Virology. 67:5617-5622 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.67.9.5617-5622.1993 |
| Popis: | Transcriptional trans-activation of the human immunodeficiency virus type 1 long terminal repeat requires that the virally encoded Tat effector interacts with its target trans-activation response element (TAR) RNA stem-loop. Although the arginine-rich region of Tat from amino acids 49 to 59 is sufficient to bind to TAR RNA in vitro, the RNA-binding domain of Tat has not been defined in vivo. Human immunodeficiency virus type 1 also encodes the Rev protein, which acts through an RNA stem-loop called the Rev-response element to transport unspliced and singly spliced viral RNA species from the nucleus to the cytoplasm. To map the RNA-binding domain of Tat, we performed assays that relied on Rev function using the heterologous RNA-tethering mechanism of Tat and the TAR. By examining the effects of selected targeted mutations of Tat on the abilities of hybrid Tat/Rev proteins to rescue the expression of unspliced mRNA via the TAR, we demonstrated that residues throughout the N-terminal 59 amino acids of Tat are required for binding of Tat and TAR RNA in vivo. |
| Databáze: | OpenAIRE |
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