Inhibitory effects of Coptidis Rhizoma on the intestinal absorption and metabolism of Scutellariae Radix
| Autor: | Ting Zhang, Zhihui Li, Guixia Sun, Pengkai Ma, Guijie Wei, Hong-Ya Wang, Chunyue Zhang, Yu-Jie Zhang, Wei Zheng, Zhe Zhang, Wen-Wen Zhao, Xiurui Sun, Jianhua Chen |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Coptis chinensis food.ingredient Metabolite Absorption (skin) Pharmacology High-performance liquid chromatography Intestinal absorption Rats Sprague-Dawley Feces 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine food Tandem Mass Spectrometry Drug Discovery Animals Glycosides Chromatography High Pressure Liquid 030304 developmental biology Flavonoids chemistry.chemical_classification 0303 health sciences Plant Extracts Chemistry Glycoside Metabolism Gastrointestinal Contents In vitro Gastrointestinal Microbiome Intestinal Absorption 030220 oncology & carcinogenesis Herb Drugs Chinese Herbal Scutellaria baicalensis |
| Zdroj: | Journal of Ethnopharmacology. 270:113785 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2021.113785 |
| Popis: | Ethnopharmacological relevance The Scutellariae Radix (SR) and Coptidis Rhizoma (CR) herb couple is widely used in traditional Chinese medicine prescriptions for the treatment of diabetes mellitus due to its interaction and synergistic effect compared to either herb alone, but the underlying mechanism of interaction between these herbs is unclear. This study aimed to investigate the effects of CR on the metabolism and absorption of SR. Materials and methods After rats were treated with normal saline (NS group) or the CR extract (CR-treated group) for seven consecutive days, the intestinal flora was extracted from rat faeces for a co-incubation with the SR extract to investigate the metabolism of SR flavonoids, and a non-everted gut sac was prepared in vitro to evaluate the intestinal absorption of the SR extract. The components of the SR extract, the metabolites of the SR extract that was co-incubated with intestinal flora, and the dialysate acquired from non-everted gut sacs were identified and determined by an HPLC-MS/MS method. The absorption rate constant (Ka) and the apparent permeability (Papp) of each compound were calculated, and the effects of CR on the metabolism and absorption of flavonoids in SR were evaluated, by comparison the Ka and Papp between two groups using Student's t-test. Results Twenty-nine flavonoids were detected and identified in the SR extract, including 16 glycosides and 13 aglycones. In the co-incubation with the intestinal flora, differences in metabolite classes were not observed between the NS group and CR-treated group; however, the metabolic rates of 17 flavonoids in the CR-treated group were significantly higher than the NS group. The Papp of 11 compounds (4 glycosides and 7 aglycones) across the gut sac were greater than 2 × 10−5 cm/s in both groups, while the Papp values of 7 compounds including wogonoside (WG) and other aglycones were significantly decreased in the CR-treated group. Conclusion Based on these results, CR decreased the metabolism and absorption of SR flavonoids, and exerted much greater inhibitory effects on aglycones than glycosides, which may be one of the potential mechanisms underlying the therapeutic effects of the combination of SR and CR on diabetes mellitus. |
| Databáze: | OpenAIRE |
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