Evidence of HIF-1 functional binding activity to caspase-3 promoter after photothrombotic cerebral ischemia
| Autor: | Michaël Van Hoecke, Christine Marie, Alain Beley, Nathalie Bertrand, Anne Prigent-Tessier, Ali Bettaieb, Rodolphe Filomenko, Philippe Garnier |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Telencephalon Caspase 3 Biology Gene Expression Regulation Enzymologic Brain ischemia Cellular and Molecular Neuroscience Hypoxia-Inducible Factor 1-Alpha medicine Animals RNA Messenger Rats Wistar Promoter Regions Genetic Molecular Biology Gene Transcription factor Regulation of gene expression Messenger RNA Binding Sites Promoter Cell Biology medicine.disease Hypoxia-Inducible Factor 1 alpha Subunit Molecular biology Rats Enzyme Activation Oxygen Disease Models Animal Hypoxia-Ischemia Brain Intracranial Thrombosis Protein Binding |
| Zdroj: | Molecular and cellular neurosciences. 34(1) |
| ISSN: | 1044-7431 |
| Popis: | Hypoxia-inducible factor 1 alpha (HIF-1alpha) is a transcription factor that was suggested in vitro to promote cell death by modulation of proapoptotic genes. In this report, we tested the hypothesis of an in vivo proapoptotic role of HIF-1alpha after an ischemic insult. For this purpose, HIF-1alpha and procaspase-3 mRNA and protein expressions were examined in rat brain subjected to 12- and 24-h permanent focal ischemia and the presence of an HIF-1 binding activity to the caspase-3 gene promoter was explored. The results showed that HIF-1alpha and procaspase-3 expressions increased with a similar pattern in response to ischemia. In addition, caspase-3 activation was observed in cells that express HIF-1alpha. Moreover, electrophoretic mobility assay revealed a specific HIF-1 binding activity to the caspase-3 gene promoter. Altogether the present data provide strong arguments for a causative relationship between HIF-1alpha and caspase-3 inductions through a functional binding activity to the caspase-3 gene promoter. |
| Databáze: | OpenAIRE |
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