Leishmania amazonensis and Leishmania chagasi: In vitro leishmanicide activity of Virola surinamensis (rol.) warb

Autor: Helliton Patrick Cordovil Brigido, Maria Fâni Dolabela, João Victor da Silva e Silva, Fernando Tobias Silveira, Marliane Batista Campos, Kelly Cristina Oliveira de Albuquerque, Maria Elinete Corrêa, Andreza do Socorro Silva da Veiga, Lourivaldo S. Santos
Rok vydání: 2016
Předmět:
Magnetic Resonance Spectroscopy
030231 tropical medicine
Immunology
Virola surinamensis
Leishmania mexicana
Ethyl acetate
Antiprotozoal Agents
Anisoles
Sesquiterpene
01 natural sciences
Lignans
Leishmania infantum / efeitos de drogas
03 medical and health sciences
chemistry.chemical_compound
Inhibitory Concentration 50
Extratos Vegetais / farmacologia
0302 clinical medicine
Column chromatography
Macr?fagos Peritoneais / parasitologia
Viability assay
Leishmania infantum
Espectroscopia de Resson?ncia Magn?tica
Nerolidol
Antiprotozo?rios / farmacologia
Chromatography
Virola
biology
Plant Extracts
General Medicine
Leishmania mexicana / efeitos de drogas
biology.organism_classification
Leishmania
0104 chemical sciences
010404 medicinal & biomolecular chemistry
Infectious Diseases
Myristicaceae / qu?mica
chemistry
Biochemistry
Macr?fagos Peritoneais / efeitos de drogas
Macrophages
Peritoneal
Parasitology
Myristicaceae
Zdroj: Repositório Digital do Instituto Evandro Chagas (Patuá)
Instituto Evandro Chagas (IEC)
instacron:IEC
ISSN: 1090-2449
Popis: Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico, Comiss?o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) and Funda??o ao Amaz?nia de Amparo a Estudos e Pesquisas (FAPESPA) for providing financial support for this study. Universidade Federal do Para. Instituto de Ci?ncias da Sa?de. Programa de P?s-Gradua??o em Inova??o Farmac?utica. Bel?m, PA, Brazil. Universidade Federal do Par?. Instituto de Ci?ncias da Sa?de. Programa de P?s-Gradua??o em Ci?ncias Farmac?uticas. Bel?m, PA, Brazil. Universidade Federal do Par?. Instituto de Ci?ncias da Sa?de. Programa de P?s-Gradua??o em Ci?ncias Farmac?uticas. Bel?m, PA, Brazil. Universidade Federal do Par?. Instituto de Ci?ncias da Sa?de. Programa de P?s-Gradua??o em Ci?ncias Farmac?uticas. Bel?m, PA, Brazil. Universidade Federal do Par?. Instituto de Ci?ncias da Sa?de. Programa de P?s-Gradua??o em Ci?ncias Farmac?uticas. Bel?m, PA, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Universidade Federal do Par?. Instituto de Ci?ncias da Sa?de. Programa de P?s-Gradua??o em Ci?ncias Farmac?uticas. Bel?m, PA, Brazil / Universidade Federal do Par?. Faculdade de Qu?mica. Bel?m, PA, Brazil. Universidade Federal do Para. Instituto de Ci?ncias da Sa?de. Programa de P?s-Gradua??o em Inova??o Farmac?utica. Bel?m, PA, Brazil / Universidade Federal do Par?. Instituto de Ci?ncias da Sa?de. Programa de P?s-Gradua??o em Ci?ncias Farmac?uticas. Bel?m, PA, Brazil. Virola species have been used in traditional medicine as healing in skin infections. From V. surinanensis oil were isolated several sesquiterpene as the nerolidol which showed activity against species of Leishmania. The current study aimed to evaluate the leishmanicide activity and toxicity of extracts, fractions and surinamesin obtained from leaves of Virola surinamensis. Hexane, Ethyl Acetate, and Methanol extracts were obtained from powder of dry leaves of V. surinamensis. The hexane and ethyl acetate extracts were fractionated by silica gel column chromatography and increasingly polar gradient. The viability of L. chagasi and L. amazonensis promastigotes was assessed by tetrazolium salt assay (MTT). Peritoneal macrophages were exposed to L. amazonensis promastigotes. The treatment was performed with the extracts for 24 h. Then, the coverslips were stained and the infection index was determined. Cytotoxicity was determined in macrophage cells by peritoneum viability assay (MTT). The selectivity index was calculated as the product of cytotoxic concentration 50% and inhibitory concentration 50%. The hexane extract showed leishmanicide activity in promastigotes. The ethyl acetate, methanol extracts and fractions (C1-C6), were inactive against promastigote form of L. chagasi and L. amzazonensis. None extract showed effect on L. amazonensis amastigotes. All samples tested showed low cytotoxicity (CC50 > 500 ?g/mL). The selectivity index of the hexane extract was greater than 5. The hexane extract of V. surinamensis was active against L. chagasi and L. amazonensis promastigotes. The extract fractionation did not increase significantly its antipromastigote activity. The surinamensin is probably not responsible for the activity. The extracts were inactive against amastigotes of L. amazonensis.
Databáze: OpenAIRE
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