Preparation of highly pure zeaxanthin particles from sea water-cultivated microalgae using supercritical anti-solvent recrystallization
| Autor: | Siang-En Hong, Chao-Rui Chen, Shih-Lan Hsu, Daina Hsiang, Yuan-Chuen Wang, Chieh-Ming J. Chang |
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| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
Environmental Engineering Chromatography Recrystallization (geology) Renewable Energy Sustainability and the Environment Elution Bioengineering General Medicine Fractionation Xanthophylls Supercritical fluid Zeaxanthin chemistry.chemical_compound chemistry Zeaxanthins Xanthophyll Solvents Seawater Response surface methodology Crystallization Waste Management and Disposal Stramenopiles |
| Zdroj: | Bioresource technology. 104 |
| ISSN: | 1873-2976 |
| Popis: | Xanthophylls, including zeaxanthin, are considered dietary supplements with a potentially positive impact on age-related macular degeneration. Using pilot-scale column fractionation coupled with supercritical anti-solvent (SAS) recrystallization, highly pure zeaxanthin particulates were prepared from ultrasonic extracts of the microalgae, Nannochloropsis oculata , grown in sea water. Column partition chromatography increased the concentration of zeaxanthin from 36.2 mg/g of the ultrasonic extracts to 425.6 mg/g of the collected column fractions. A response surface methodology was systematically designed for the SAS process by changing feed concentration, CO 2 flow rate and anti-solvent pressure. Zeaxanthin-rich particles with a purity of 84.2% and a recovery of 85.3% were produced using supercritical anti-solvent recrystallization from the column eluate at a feed concentration of 1.5 mg/mL, CO 2 flow rate of 48.6 g/min and pressure of 135 bar. |
| Databáze: | OpenAIRE |
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