Schistosoma mansoni excretory circulating cathodic antigen shares Lewis-x epitopes with a human granulocyte surface antigen and evokes host antibodies mediating complement-dependent lysis of granulocytes
Autor: | GJ van Dam, FH Claas, M Yazdanbakhsh, YC Kruize, AC van Keulen, ST Ferreira, JP Rotmans, AM Deelder |
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Rok vydání: | 1996 |
Předmět: |
Immunology
Molecular Mimicry Antibodies Helminth Antibodies Monoclonal Lewis X Antigen Cell Biology Hematology Complement System Proteins Helminth Proteins Schistosoma mansoni Cross Reactions Biochemistry Epitopes Mice Immunoglobulin M Antibody Specificity Antigens Helminth parasitic diseases Animals Humans Glycoproteins Granulocytes |
Zdroj: | Blood. 88(11) |
ISSN: | 0006-4971 |
Popis: | Parasitic worms of the genus Schistosoma excrete relatively large amounts of immunogenic glycoproteins (circulating cathodic antigen [CCA]) that contain polysaccharide side chains with the trisaccharide Lewis-x (L(ex)) as a repeating unit. These carbohydrates evoke high titers of specific IgM antibodies that cross-react with the repeating L(ex) units on the surface of granulocytes. Consequently this might lead, in the presence of complement, to lysis of the granulocytes. In the present study, this hypothesis was investigated using anti-CCA mouse monoclonal antibodies (MoAbs) and polyclonal antibodies purified from sera of infected humans. By flow cytometry, it was demonstrated that the mouse MoAbs directed against CCA strongly recognized the granulocytes. It could also be shown that these MoAbs, as well as anti- CCA IgM antibodies purified from infected human sera, caused lysis of granulocytes in a complement-dependent cytotoxicity assay. Sera from healthy controls or from patients with other helminth infections resulted in negligible granulocytotoxicity. These in vitro observed phenomena may explain the mild to moderate neutropenia that occurs in schistosomiasis patients. |
Databáze: | OpenAIRE |
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