Grape seed proanthocyanidin extract attenuates oxidant injury in cardiomyocytes
| Autor: | Kim Wojcik, Paul T. Schumacker, Lance B. Becker, Danhong Zhao, Yimin Qin, Lucy Dey, Chun-Su Yuan, Travis Anderson, Zuo-Hui Shao, Chang Qing Li, Terry L. Vanden Hoek |
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| Rok vydání: | 2003 |
| Předmět: |
Potassium Channels
Cell Survival Antimycin A Chick Embryo In Vitro Techniques Nitric Oxide chemistry.chemical_compound Superoxides Animals Myocytes Cardiac Proanthocyanidins Vitis Propidium iodide Cells Cultured Protein Kinase C Protein kinase C Flavonoids Pharmacology chemistry.chemical_classification Reactive oxygen species biology Plant Extracts Superoxide Membrane Proteins Hydrogen Peroxide Oxidants Cytoprotection Nitric oxide synthase Oxidative Stress chemistry Biochemistry Seeds biology.protein Hydroxyl radical Reactive Oxygen Species |
| Zdroj: | Pharmacological Research. 47:463-469 |
| ISSN: | 1043-6618 |
| Popis: | This study sought to test whether grape seed proanthocyanidin extract (GSPE) attenuates exogenous and endogenous oxidant stress induced in chick cardiomyocytes and whether this cytoprotection is mediated by PKC activation, mito K(ATP) channel opening, NO production, oxidant scavenging, or iron chelating effects. Cells were exposed to hydrogen peroxide (H(2)O(2)) (exogenous oxidant stress, 0.5mM) or antimycin A (endogenous oxidant stress, 100 micro M) for 2h following pretreatment with GSPE at various concentrations for 2h. Cells were also pretreated with GSPE or with inhibitors of PKC (chelerytherine), mito K(ATP) channel (5-hydroxydecanoate), nitric oxide synthase (nitro-L-arginine methyl ester) for 2h. Oxidant stress was measured by 2',7'-dichlorofluorescin diacetate and cell viability was assessed using propidium iodide. Free radical scavenging and iron chelating ability was tested in vitro. GSPE dose-dependently attenuated oxidant formation and significantly improved cell survival and contractile function. However, inhibitors of PKC, mito K(ATP) channel or NO synthase failed to abolish the protective action of GSPE during H(2)O(2) or antimycin A exposure. In vitro studies suggested that GSPE scavenges H(2)O(2), hydroxyl radical and superoxide, and may chelate iron. These results indicate that GSPE confers cardioprotection against exogenous H(2)O(2)- or antimycin A-induced oxidant injury. Its effect does not require PKC, mito K(ATP) channel, or NO synthase, presumably because it acts by reactive oxygen species scavenging and iron chelating directly. |
| Databáze: | OpenAIRE |
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