Identification of estrogen-regulated genes during fracture healing, using DNA microarray
| Autor: | Herrick J. Siegel, Hiroshi Hatano, Mark E. Bolander, Gobinda Sarkar, James T. Bronk, Hiroshi Yamagiwa, Russell T. Turner |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class Ovariectomy Endocrinology Diabetes and Metabolism Down-Regulation Bone healing Biology Fractures Bone Endocrinology Internal medicine Gene expression medicine Animals Orthopedics and Sports Medicine RNA Messenger Bony Callus Gene Oligonucleotide Array Sequence Analysis Expressed Sequence Tags Fracture Healing Messenger RNA Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Reproducibility of Results Estrogens General Medicine Urokinase-Type Plasminogen Activator Rats Gene expression profiling Reverse transcription polymerase chain reaction Disease Models Animal Estrogen Ovariectomized rat Female |
| Zdroj: | Journal of Bone and Mineral Metabolism. 22:224-235 |
| ISSN: | 1435-5604 0914-8779 |
| DOI: | 10.1007/s00774-003-0482-y |
| Popis: | Estrogen deficiency impairs fracture healing, while estrogen treatment of ovariectomized rats accelerates fracture healing. To identify genes regulated by estrogen during fracture healing, we evaluated gene expression in calluses from three groups of rats: sham-operated, ovariectomized, and ovariectomized and treated with estrogen. RNA from calluses harvested 10 days after fracture was subjected to DNA microarray-based analysis of 5147 genes. In total, 52 genes were identified whose mRNA expressions were found to be downregulated by ovariectomy but restored with estrogen. Differential mRNA expression of 4 genes (collagen type 2, extracellular superoxide dismutase, urokinase-type plasminogen activator [ u-PA], and ptk-3) was confirmed by reverse transcription polymerase chain reaction. Further, chondrocytes and chondroclasts were positive for u-PA in the junction between cartilage and bone, implying its importance in resorption and remodeling of callus. Identification of these genes is a prerequisite to understanding the mechanism by which estrogen influences the complex metabolic process of fracture repair. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink