HPLC assisted chemobiological standardization of alpha-glucosidase-I enzyme inhibitory constituents from Piper longum Linn-An Indian medicinal plant
| Autor: | Anuradha Vummenthula, Madhusudana Rao Janaswamy, Hari B. Tatipaka, Krishna Rao Dasari, Ashok Kumar Tiwari, Umamaheswara Sarma Vanka, Srinivas V. Pullela, Ikhlas A. Khan |
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| Rok vydání: | 2006 |
| Předmět: |
India
Pharmacognosy High-performance liquid chromatography chemistry.chemical_compound Sesamin Drug Discovery Glycoside Hydrolase Inhibitors Enzyme Inhibitors Chromatography High Pressure Liquid Pharmacology Detection limit Lignan Active ingredient Piper Chromatography Plants Medicinal biology Chemistry Plant Extracts Reproducibility of Results alpha-Glucosidases Piperaceae Reference Standards biology.organism_classification Medicine Ayurvedic Calibration |
| Zdroj: | Journal of ethnopharmacology. 108(3) |
| ISSN: | 0378-8741 |
| Popis: | Formulations of traditional medicines are usually made up of complex mixture of herbs. However, effective quality control methods in order to select right quality materials are lacking. Though Piper longum is a widely used herb in several Ayurvedic formulations prescribed for various diseases, there is no analytical method in the literature so far which can help in selecting the right quality material with proper proportions of the active ingredients (α-glucosidase-I enzyme inhibitory principles). We employed a systematic bioassay guided fractionation method and isolated pipataline, pellitorine, sesamin, brachystamide B and guineensine as active principles. A reversed-phase high-performance liquid chromatography method was developed to quantify these active principles in the plant material, which can serve as an effective quality control tool. The separation was carried out using a Discovery HS F5 C-18 (ODS) column and the solvent system used was a gradient comprising of (A) acetonitrile and (B) water with a flow rate of 1 ml/min. The detection was performed using a PDA detector. Regression equation pertaining to all the bioactive isolates revealed a linear relationship (r2 > 0.9995). The detection limits (S/N = 3) ranged from 0.005 to 0.001 μg/ml. Of all the active isolates, sesamin was identified to be present in maximum quantities (0.91%) where as brachystamide B was found in minimum quantity (0.01%). |
| Databáze: | OpenAIRE |
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