Bacterioruberin extracts from a genetically modified hyperpigmented Haloferax volcanii strain: antioxidant activity and bioactive properties on sperm cells
| Autor: | Lucia Zalazar, R. E. De Castro, Carmen María Lacasa Martínez, Ana J. Soler, P. Pagola, Micaela Cerletti, María Sandra Churio, María Iniesta-Cuerda, M. V. Miró, Andreina Cesari |
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| Přispěvatelé: | Agencia Nacional de Promoción Científica y Tecnológica (Argentina), Universidad Nacional de Mar del Plata |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
Antioxidant Biotecnology medicine.medical_treatment GENETIC Ram Sperm Cells Applied Microbiology and Biotechnology Cryopreservation Antioxidants Ciencias Biológicas 03 medical and health sciences Biología Celular Microbiología beta-Carotene medicine Genetics Animals BIOTECHNOLOGY Viability assay Food science Carotenoid Haloferax volcanii 030304 developmental biology chemistry.chemical_classification 0303 health sciences Sheep biology 030306 microbiology Haloferax Volcanii General Medicine biology.organism_classification Sperm Carotenoids Spermatozoa chemistry Haloarchaea Sperm Motility CIENCIAS NATURALES Y EXACTAS Biotechnology Bacterioruberin |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| DOI: | 10.1111/jam.14160 |
| Popis: | [Aims]: To examine the antioxidant activity of Bacterioruberin (Bctr)‐rich extracts isolated from a hyperpigmented, genetically modified Haloferax volcanii strain (HVLON 3) and to investigate the effect on cold‐sensitive ram sperm cells. [Methods and Results]: The strain HVLON 3 produces higher Bctr amounts than most haloarchaea (220 ± 13 mg g−1 DW ). HVLON 3‐Bctr extract has higher antioxidant activity than β‐carotene (threefold) as evaluated using 2,2 diphenyl‐1‐picrylhydrazyl combined with Electron Paramagnetic Resonance analysis (EC 50 4·5 × 10−5 mol l−1 vs 13·9 × 10−5 mol l−1 respectively). Different concentrations of HVLON 3‐Bctr extracts were assayed on ram sperm after freezing/thawing and physiologically relevant parameters were examined. Extracts containing 7 and 20 μ mol l−1 Bctr significantly improved cell viability (P < 0·0001), total and progressive motility (P < 0·0001) and sperm velocities (P = 0·0172 for curvilinear velocity VCL , P = 0·0268 for average path velocity VAP and P = 0·0181 for straight line velocity VSL ) and did not affect other parameters evaluated. [Conclusions]: HVLON 3 is an excellent source of natural microbial C50 carotenoids with applicability in Biotechnology, Biomedical and Veterinary fields. HVLON 3 Bctr extract improves the quality of cryopreserved ram sperm cells and could be applied to increase insemination yields. [Significance and Impact of the Study]: This study provides an insight on the bioactive properties of a bioproduct derived from haloarchaea (carotenoids) which are so far underexploited. This research was supported by the National Agency for Scientific and Technological Promotion (ANPCyT) grants PICT‐2015‐3682 and PICT‐2014‐1477 awarded to A.C. and R.D.C., respectively; UNMDP grants 15/E818, EXA819/17 and EXA823/17 awarded to M.S.C., A.C. and R.D.C, respectively. |
| Databáze: | OpenAIRE |
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