Monitoring the refractive index of tissue models using light scattering spectroscopy
| Autor: | Nicholas Stone, Benjamin Gardner, Martina Alunni-Cardinali, Francesca Palombo, Michelle Bailey, Silvia Caponi, Daniele Fioretto |
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| Rok vydání: | 2020 |
| Předmět: |
Raman scattering
Materials science Light Calibration curve Infrared spectroscopy FOS: Physical sciences 02 engineering and technology Spectrum Analysis Raman Brillouin microscopy Vibration biomechanics 03 medical and health sciences symbols.namesake Biopolymers Optics Physics - Biological Physics Physics::Atomic Physics Raman Instrumentation Spectroscopy 030304 developmental biology 0303 health sciences business.industry Spectrum Analysis Hydrogels Articles 021001 nanoscience & nanotechnology vibrational spectroscopy 3. Good health Refractometry Biological Physics (physics.bio-ph) Molecular vibration Self-healing hydrogels symbols 0210 nano-technology business Raman spectroscopy Refractive index |
| Zdroj: | Applied Spectroscopy |
| DOI: | 10.48550/arxiv.2009.02274 |
| Popis: | In this work, we report the application of Raman microspectroscopy for analysis of the refractive index of a range of tissue phantoms. Using both a custom-developed setup with visible laser source and a commercial micro-spectrometer with near infrared laser, we measured the Raman spectra of gelatin hydrogels at various concentrations. By building a calibration curve from measured refractometry data and Raman scattering intensity for different vibrational modes of the hydrogel, we determined the refractive indices of the gels from their Raman spectra, with approximately the same accuracy as that of refractometry measurements with an Abbe refractometer. This work highlights the importance of a correlative approach through Brillouin-Raman microspectroscopy for the mechano-chemical analysis of biologically relevant samples. Comment: 11 pages, 5 figures, 1 table |
| Databáze: | OpenAIRE |
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