Online Liquid Microextraction Coupled with HPLC-ABTS for Rapid Screening of Natural Antioxidants: Case Study of Three Different Teas
| Autor: | Chun-Hong Li, Huang Qi, Hai-Ming Chen, Wen-Jia Li, Zheng-Ming Qian, Ling Chen, Bo-Wen Fang, De-qiang Li |
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| Rok vydání: | 2020 |
| Předmět: |
ABTS
Chromatography Tea Liquid Phase Microextraction Formic acid Extraction (chemistry) Reproducibility of Results Catechin General Medicine High-performance liquid chromatography Antioxidants Camellia sinensis Analytical Chemistry chemistry.chemical_compound Rutin chemistry Chlorogenic acid Gallocatechin Benzothiazoles Sulfonic Acids Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatographic Science. 58:875-879 |
| ISSN: | 1945-239X 0021-9665 |
| DOI: | 10.1093/chromsci/bmaa047 |
| Popis: | In the present study, an online liquid extraction coupled with high-performance liquid chromatography-2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (HPLC-ABTS) system for rapid screening of antioxidants in tea samples was proposed. As an example, the tea samples were firstly extracted by online HPLC extractor with mobile phase at 70°C, then the hyphenated HPLC-ABTS was used for the chromatographic separation on a Poroshell EC C18 column by 0.3% aqueous formic acid and acetonitrile with a gradient elution at 1.5 mL·min−1, and the UV and antioxidant chromatograms with detection wavelengths at 270 nm and 750 nm were recorded, respectively. The established system integrated the processes of online HPLC sample extraction, HPLC separation and online antioxidants detection, the total analysis time of which was |
| Databáze: | OpenAIRE |
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