Naturally occurring hypoallergenic Bet v 1 isoforms fail to induce IgE responses in individuals with birch pollen allergy

Autor: Stefan Vieths, Karin Hoffmann-Sommergruber, Lothar Vogel, Christian Radauer, Stefan Wagner, Tamara Kopp, Merima Bublin, Heimo Breiteneder, Otto Scheiner, Elli K. Greisenegger
Rok vydání: 2008
Předmět:
Zdroj: Journal of Allergy and Clinical Immunology. 121:246-252
ISSN: 0091-6749
DOI: 10.1016/j.jaci.2007.08.006
Popis: Background Engineered hypoallergens are currently being investigated for specific immunotherapy of allergic diseases in preclinical and clinical studies. Naturally occurring hypoallergens have by and large not been considered as a source of vaccine candidates. Objective Evaluation of the antibody response in atopic individuals induced by birch pollen containing isoforms of the major birch pollen allergen Bet v 1. Methods Isoform-specific antibody isotype responses for Bet v 1.0101, Bet v 1.0401, and Bet v 1.1001 were determined for 35 sera of individuals with birch pollen allergy. Isoform structures were compared and related to IgE-binding inhibitory capacities and induction of mediator release in human Fcɛ receptor transformed rat basophilic leukemia cells. Results Bet v 1.0101 induced a predominant IgE response, whereas the significant highest levels of IgG 4 antibodies were directed against Bet v 1.0401. Bet v 1.1001 induced only a minimal antibody response. Structural comparisons revealed that most of the amino acid differences between the isoforms were located on the protein surfaces. IgE induced by Bet v 1.0101 only partly cross-reacted with the 2 other isoforms and bound to them with notably lower affinity. Bet v 1.0401 and Bet v 1.1001 also were poor inducers of mediator release. Conclusion Bet v 1 isoforms possess highly variant immunogenic and allergenic properties. Bet v 1.0101 acts as the sensitizing agent, whereas Bet v 1.0401 and Bet v 1.1001 can induce only a minimal IgE response.
Databáze: OpenAIRE
Externí odkaz: