Autologous adipose-derived mesenchymal stem cells and hydroxyapatite for bone defect in rabbits

Autor:	P. F. Malard, Fyk Kawamoto, Livia de Paula Coelho, BM Alcantara, Guilherme Galhardo Franco, ER Carvalho, Bruno Watanabe Minto, L.G.G.G. Dias
Přispěvatelé:	Universidade Estadual Paulista (UNESP), BioCell
Rok vydání:	2022
Předmět:	Pathology medicine.medical_specialty General Veterinary Chemistry Mesenchymal stem cell medicine Adipose tissue Fracture healing Cell transplantation Adipose derived MSC Bone defect Oryctolagus cuniculus
Zdroj:	Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP
ISSN:	1805-9392 0375-8427
DOI:	10.17221/85/2020-vetmed
Popis:	Made available in DSpace on 2022-04-28T19:48:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01 This study aims to evaluate the effect of autologous adipose-derived mesenchymal stem cells (AAD-MSC), with and without synthetic absorbable hydroxyapatite (HAP-91), on the bone regeneration in rabbits. Thirty-four female white New Zealand rabbits were submitted to a 10 mm distal diaphyseal radius ostectomy, divided into 3 experimental groups according to the treatment established. The bone gap was filled with 0.15 ml of a 0.9% saline solution containing two million AAD-MSC (G1), or AAD-MSC associated with HAP-91 (G2). The control group (CG) received only 0.15 ml of the 0.9% saline solution. Radiographs were made post-operatively, and after 15, 30, 45 and 90 days. Fifty percent of the samples were submitted to a histological examination at 45 days and the remaining ones at 90 days post-operatively. Radiographically, the periosteal reaction, bone callus volume and bone bridge quality were superior in G2 (P < 0.05). Histologically, the bone repair was faster and more efficient in G1 at 45 days (P < 0.05). In conclusion, AAD-MSC improved the regeneration on the experimentally induced bone defects in rabbits; however, the use of hydroxyapatite requires caution given the granulomatous reaction produced in the species. Department of Clinical and Veterinary Surgery Faculty of Agrarian and Veterinary Sciences São Paulo State University (UNESP), SP BioCell, DF Department of Clinical and Veterinary Surgery Faculty of Agrarian and Veterinary Sciences São Paulo State University (UNESP), SP
Databáze:	OpenAIRE
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