Developmentally regulated alterations in Polycomb repressive complex 1 proteins on the inactive X chromosome
| Autor: | Kathrin Plath, Karien M. Hamer, Barbara Panning, Thomas P. Yang, Dale Talbot, Rudolf Jaenisch, Arie P. Otte |
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| Přispěvatelé: | Epigenetic Regulation of Gene Expression (inactive) (SILS, FNWI) |
| Rok vydání: | 2004 |
| Předmět: |
X Chromosome
Macromolecular Substances 1.1 Normal biological development and functioning Polycomb-Group Proteins macromolecular substances Methylation Medical and Health Sciences Article X-inactivation Cell Line Histones Mice Histone H3 Species Specificity Genetic Underpinning research Dosage Compensation Genetic Genetics Polycomb-group proteins Animals Humans Drosophila Proteins Epigenetics Research Articles Polycomb Repressive Complex 1 Dosage compensation biology Lysine Cell Biology Biological Sciences Molecular biology Repressor Proteins Kinetics Histone Dosage Compensation biology.protein Drosophila Female XIST Generic health relevance Carrier Proteins PRC2 Developmental Biology |
| Zdroj: | The Journal of cell biology, vol 167, iss 6 Journal of Cell Biology, 167, 1025-1035. Rockefeller University Press The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.200409026 |
| Popis: | Polycomb group (PcG) proteins belonging to the polycomb (Pc) repressive complexes 1 and 2 (PRC1 and PRC2) maintain homeotic gene silencing. In Drosophila, PRC2 methylates histone H3 on lysine 27, and this epigenetic mark facilitates recruitment of PRC1. Mouse PRC2 (mPRC2) has been implicated in X inactivation, as mPRC2 proteins transiently accumulate on the inactive X chromosome (Xi) at the onset of X inactivation to methylate histone H3 lysine 27 (H3-K27). In this study, we demonstrate that mPRC1 proteins localize to the Xi, and that different mPRC1 proteins accumulate on the Xi during initiation and maintenance of X inactivation in embryonic cells. The Xi accumulation of mPRC1 proteins requires Xist RNA and is not solely regulated by the presence of H3-K27 methylation, as not all cells that exhibit this epigenetic mark on the Xi show Xi enrichment of mPRC1 proteins. Our results implicate mPRC1 in X inactivation and suggest that the regulated assembly of PcG protein complexes on the Xi contributes to this multistep process. |
| Databáze: | OpenAIRE |
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