Immobilization of Saccharomyces cerevisiae on to radiation crosslinked HEMA/AAm hydrogels for production of ethyl alcohol

Autor: A. Yasemin Öztop, Yasemin Işıkver, Dursun Saraydin, H. Nursevin Öztop
Přispěvatelé: Cumhuriyet Univ, Dept Chem, TR-58140 Sivas, Turkey -- Cumhuriyet Univ, Fac Med, Microbiol & Clin Microbiol Dept, TR-58140 Sivas, Turkey, Saraydin, Dursun -- 0000-0001-7191-510X
Rok vydání: 2002
Předmět:
Zdroj: Process Biochemistry. 37:651-657
ISSN: 1359-5113
DOI: 10.1016/s0032-9592(01)00254-0
Popis: WOS: 000173977500012
Radiation crosslinked 2-hydroxyethyl methacrylate/acrylamide (HEMA/AAm) copolymers prepared by gamma-irradiation were used in experiments on swelling, diffusion, immobilization of yeast cells (Saccharomyces cerevisiae) and production of ethyl alcohol. HEMA/AAm hydrogels irradiated at 3 kGy were used for swelling and diffusion studies in water and the nutrient medium of the cells. The parameters of equilibrium swelling, maximum swelling, initial swelling rate, diffusional exponent, network constant and diffusion coefficient of the hydrogel/penetrant systems were calculated and evaluated. All parameters for the hydrogel/water system were higher than that of the hydrogel/nutrient medium systems. Yeast cells (Saccharomyces cerevisiae) were immobilized on to the hydrogels by adsorption during multiplication and ethyl alcohol production of the hydrogels was investigated. Swelling of HEMA increased with the addition of AAm and ethyl alcohol production increased with increasing AAm in the hydrogels. (C) 2002 Elsevier Science Ltd. All rights reserved.
Databáze: OpenAIRE