Sanguisorba officinalis L. suppresses 5-fluorouracil-sensitive and-resistant colorectal cancer growth and metastasis via inhibition of the Wnt/β-catenin pathway
| Autor: | Yanjun Hong, Cheng Jiang, Jiahui Yan, Pengting Chen, Yuemei Yuan, Meicun Yao, Chang Peng, Wei-Jia Zhang, Shuyi Sang |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Epithelial-Mesenchymal Transition
Cell Mice Nude Pharmaceutical Science Sanguisorba Metastasis Mice Downregulation and upregulation Cell Movement Cell Line Tumor Drug Discovery medicine Animals Humans Wnt Signaling Pathway beta Catenin Cell Proliferation Pharmacology Chemistry Cell growth Wnt signaling pathway Cell migration medicine.disease medicine.anatomical_structure Complementary and alternative medicine Apoptosis Catenin Cancer research Molecular Medicine Fluorouracil Colorectal Neoplasms |
| Zdroj: | Phytomedicine. 94:153844 |
| ISSN: | 0944-7113 |
| Popis: | Background Colorectal cancer (CRC) is a widespread cancer with high morbidity and mortality. Chemoresistance and metastasis are the current challenges for CRC treatment. Sanguisorba officinalis Linn. (called DiYu in Chinese, DY) is a Traditional Chinese medicine (TCM), which its root is long used as medicinal part. In our previous study, the aqueous extract of DY could inhibit the Wnt/β-catenin pathway and showed great antitumor effect against CRC. The Wnt/β-catenin pathway is involved in CRC chemoresistance and metastasis. However, there is little study on the antitumor and antimetastatic effects of DY on resistant CRC cells. The aim of this study is to explore the effect of aqueous extract of DY on the growth and metastasis of 5-fluorouracil (5-FU) sensitive and resistant CRC, and to elucidate the underlying molecular mechanism. Methodology In this study, cell viability, cell colony formation and apoptosis analyses were performed to verify the in vitro antitumor effect of DY on 5-FU-sensitive and -resistant CRC cells. Next, transwell assays were used to test the inhibition activity of DY on CRC migration and invasion. Western Blotting assays were carried out to identify the molecular mechanism underlying the efficacy of DY extract. Xenograft CRC nude mice model and tumor metastasis model were used to confirm the in vivo antitumor and antimetastatic effects of DY. Results DY inhibited cell proliferation and apoptosis via the upregulation of Bax, cleaved-caspase3 and cleaved-PARP proteins and downregulation of Bcl-2 protein. DY also inhibited cell migration and invasion via the downregulation of N-cadherin, vimentin and snail proteins and upregulation of E-cadherin protein, demonstrating that DY suppressed cell metastasis by reversing epithelial-to-mesenchymal transition (EMT) procession. Moreover, the protein expression levels of β-catenin in whole cell, cytoplasm and nucleus were decreased after DY treatment. Taken together, DY suppressed CRC cell growth and metastasis via inhibition of the Wnt pathway. Additionally, DY also demonstrated effective antitumor and anti-metastasis activities in vivo. Conclusions In conclusion, DY suppressed the growth and metastasis of 5-FU-sensitive and -resistant CRC via inhibition of the Wnt pathway, which indicated that DY could be a potential drug to treat CRC patients and improve clinic outcome. |
| Databáze: | OpenAIRE |
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