An in vitro-identified high-affinity nucleosome-positioning signal is capable of transiently positioning a nucleosome in vivo
Autor: | Anton Valouev, Andrew Fire, Zhi-Ying Chen, Mark A. Kay, Arend Sidow, Lia Gracey, Jay M. Maniar |
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Jazyk: | angličtina |
Rok vydání: | 2010 |
Předmět: |
Genetics
0303 health sciences lcsh:QH426-470 Research Eukaryotic DNA replication Context (language use) Biology Genome DNA sequencing Cell biology Chromatin 03 medical and health sciences chemistry.chemical_compound lcsh:Genetics 0302 clinical medicine chemistry In vivo Nucleosome Molecular Biology 030217 neurology & neurosurgery DNA 030304 developmental biology |
Zdroj: | Epigenetics & Chromatin, Vol 3, Iss 1, p 13 (2010) Epigenetics & Chromatin |
ISSN: | 1756-8935 |
Popis: | Background The physiological function of eukaryotic DNA occurs in the context of nucleosomal arrays that can expose or obscure defined segments of the genome. Certain DNA sequences are capable of strongly positioning a nucleosome in vitro, suggesting the possibility that favorable intrinsic signals might reproducibly structure chromatin segments. As high-throughput sequencing analyses of nucleosome coverage in vitro and in vivo have become possible, a vigorous debate has arisen over the degree to which intrinsic DNA:nucleosome affinities orchestrate the in vivo positions of nucleosomes, thereby controlling physical accessibility of specific sequences in DNA. Results We describe here the in vivo consequences of placing a synthetic high-affinity nucleosome-positioning signal, the 601 sequence, into a DNA plasmid vector in mice. Strikingly, the 601 sequence was sufficient to position nucleosomes during an early phase after introduction of the DNA into the mice (when the plasmid vector transgene was active). This positioning capability was transient, with a loss of strong positioning at a later time point when the transgenes had become silent. Conclusions These results demonstrate an ability of DNA sequences selected solely for nucleosome affinity to organize chromatin in vivo, and the ability of other mechanisms to overcome these interactions in a dynamic nuclear environment. |
Databáze: | OpenAIRE |
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