Anti-telomere antibodies in systemic lupus erythematosus: a new ELISA test for anti-DNA with potential pathogenetic implications
| Autor: | Salonen Em, Metzger Al, Pashinian N, Erik Avaniss-Aghajani, Morris R, Daniel J. Wallace |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Adolescent Enzyme-Linked Immunosorbent Assay 030204 cardiovascular system & hematology Sensitivity and Specificity 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Rheumatology Antibody Specificity Predictive Value of Tests immune system diseases medicine Humans Lupus Erythematosus Systemic skin and connective tissue diseases Aged Autoantibodies 030203 arthritis & rheumatology Lupus erythematosus Systemic lupus erythematosus biology medicine.diagnostic_test business.industry Middle Aged Telomere Prognosis medicine.disease 3. Good health chemistry Antibodies Antinuclear Rheumatoid arthritis Immunoassay Immunology biology.protein Antibody business DNA Anti-SSA/Ro autoantibodies |
| Zdroj: | Lupus. 9:328-332 |
| ISSN: | 1477-0962 0961-2033 |
| DOI: | 10.1191/096120300678828343 |
| Popis: | Background: Telomeric hexamer repeats (TTAGGG/CCCTAA)nare highly repetitive sequences of DNA. They cap the termini of eukaryotic chromosomes and stabilize them, preventing degradation or fusion. Anti ds-DNA is one of the most specific tests for systemic lupus erythematosus (SLE). Of related importance, a preliminary report has suggested that anti-telomere antibodies are also highly specific for the presence of SLE.Methods: 220 patients with SLE, 79 with rheumatoid arthritis (RA), 54 with other rheumatic diseases and 99 healthy controls were tested for anti-telomere antibody as measured by enzyme immunoassay detecting 30and 60-mer telomeric repeats (5 ± 10 hexamers). 48 of the 220 SLE patients charts were abstracted for 90 separate clinical, laboratory and treatment parameters. Comparisons were made between SLE and non-SLE patients, and within the lupus group for telomere positivity and among the latter 48 patients for anti-DNA (Farr) levels and SLEDAI scores.Results: Anti-telomere antibody was present in 48.6% of the overall SLE group (220), 71% of our cohort (48), 11% with primary Sjogren's (2=18), 7.6% with RA (6=79) and 2% of normal controls (2=99) (P < 0.001 comparing SLE to all other groups). In the 48 patient cohort, anti-telomere antibody was more sensitive than anti-dsDNA (Farr) (71% vs 50%), but did not correlate with other clinical parameters, SLEDAI scores, or other autoantibodies.Conclusions: The detection of anti-telomere antibody appears to be more sensitive and may be as specific as anti-dsDNA (Farr) in SLE. The detection of telomeric repeats may be as accurate as other anti-DNA assay methodologies and more specific for the presence of SLE. The immunogenic potential of telomere biology related to the pathogenesis and/or diagnosis of SLE deserves further investigation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|