Overgrowth of a leukemic culture by a minor CD34+ population
| Autor: | Paul Baines, T. Hoy, Louise N. Truran, Helen Lake, Janet Fisher, Alan Kenneth Burnett |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Cancer Research
Cellular differentiation Population Lipopolysaccharide Receptors Retinoic acid Lewis X Antigen Antigens CD34 Antigens CD7 Biology Colony-Forming Units Assay Leukocyte Count chemistry.chemical_compound Tretinoin Tumor Cells Cultured medicine Humans Progenitor cell Clonogenic assay education Aged Interleukin 3 education.field_of_study Hematology Hematopoietic Stem Cells Molecular biology CD56 Antigen Clone Cells Oncology chemistry Leukemia Myeloid Cell culture Acute Disease Immunology Leukocytes Mononuclear Leukocyte Common Antigens Cell Division medicine.drug |
| Zdroj: | Leukemia Research. 22:549-556 |
| ISSN: | 0145-2126 |
| Popis: | We have investigated the differentiation potential of blast cells in a case of acute myeloid leukemia which comprised a majority CD34- population and a minor (2%) CD34+ fraction. Blasts were cultured for 2 weeks in a combination of cytokines--c-Kit ligand, interleukin 3 and granulocyte macrophage colony-stimulating factor (SIGm mix)--together with all-trans retinoic acid or 1alpha ,25-dihydroxy vitamin D3. Maturation of blasts was assessed by morphology on Romanowsky-stained slides, changes in surface CD markers and clonogenic culture. After 7 days of culture of unseparated blasts in SIGm, most maturation was monocytic, but with retinoic acid 63% of blasts had matured into granulocytes. Vitamin D3 enhanced monocytic differentiation, with 60% of cells becoming monocytic. The percentage of CD14 and CD15 positive cells decreased over 7 days in SIGm (from 62% to 17% and from 76% to 39% for CD14 and CD15, respectively). CD14+ cell numbers were maintained, or recovered, in cultures supplemented with vitamin D3 (59% at day 7), and CD15+ cell numbers, too, remained unchanged in the presence of retinoic acid (67%) or vitamin D3 (66%). Aberrant markers CD7 and CD56 declined under any conditions. When separated, both the CD34- and CD34+ fractions showed similar changes in morphology and surface maturation markers, suggesting that these two populations may be closely related. However, only a few CD34+ cells expressed the aberrant markers present on the majority blast population. The CD34- population declined in culture while the CD34+ fraction rapidly expanded. This probably reflects the difference in progenitor content; high numbers of colony-forming cells were concentrated in the CD34+ subpopulation. We conclude that both CD34- and CD34+ populations can differentiate but only the CD34+ fraction proliferates. Primitive clonogenic CD34+ cells from this patient may generate occasional aberrant CD34+ blasts which could then differentiate into the accumulating aberrant CD34- blast population. |
| Databáze: | OpenAIRE |
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