Microarray analyses of the dorsal root ganglia support a role for innate neuro-immune pathways in persistent pain in experimental osteoarthritis
| Autor: | Anne-Marie Malfait, Rachel E. Miller, Delfien Syx, Dongjun Ren, Ana M. Valdes, S. Ishihara, Richard J. Miller, P.B. Tran |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine CCR2 Microarray Neuroimmunomodulation Biomedical Engineering Gene Expression Disease Osteoarthritis Bioinformatics Menisci Tibial Article Transcriptome Mice 03 medical and health sciences 0302 clinical medicine Rheumatology Ganglia Spinal Animals Medicine Orthopedics and Sports Medicine RNA Messenger Neuroinflammation 030203 arthritis & rheumatology Pain Postoperative business.industry Gene Expression Profiling Sham surgery Osteoarthritis Knee Microarray Analysis medicine.disease Arthralgia Arthritis Experimental Immunity Innate Phenotype 030104 developmental biology Neuropathic pain Disease Progression business |
| Zdroj: | Osteoarthritis Cartilage |
| ISSN: | 1063-4584 |
| DOI: | 10.1016/j.joca.2020.01.008 |
| Popis: | OBJECTIVE: Following destabilization of the medial meniscus (DMM), mice develop experimental osteoarthritis (OA) and associated pain behaviors that are dependent on the stage of disease. We aimed to describe changes in gene expression in knee-innervating dorsal root ganglia (DRG) after surgery, in order to identify molecular pathways associated with three pre-defined pain phenotypes: “post-surgical pain”, “early-stage OA pain”, and “persistent OA pain”. DESIGN: We performed DMM or sham surgery in 10-week old male C57BL/6 mice and harvested L3-L5 DRG 4, 8, and 16 weeks after surgery or from age-matched naïve mice (n=3/group). RNA was extracted and an Affymetrix Mouse Transcriptome Array 1.0 was performed. Three pain phenotypes were defined: “post-surgical pain” (sham and DMM 4-week vs. 14-week old naïve), “early OA pain” (DMM 4-week vs. sham 4-week), and “persistent OA pain” (DMM 8- and 16-week vs. naïve and sham 8- and 16-week). ‘Top hit’ genes were defined as p |
| Databáze: | OpenAIRE |
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