High throughput multiple locus variable number of tandem repeat analysis (MLVA) of Staphylococcus aureus from human, animal and food sources

Autor: Stefan Schwarz, Fabienne Loisy-Hamon, Gilles Vergnaud, Andrea T. Feßler, Christine Pourcel, Michaël Treilles, Daniel Sobral, Kristina Kadlec, Benoît Lebeau, Florence B. Gilbert, Anne Brisabois, Dominique Bergonier
Přispěvatelé: Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), European Defense Agency, German Federal Ministry of Education, Research (BMBF) through the German Aerospace Center (DLR) [01KI1014D], association Vaincre La Mucoviscidose [RC0630], French Direction Generale de l'Armement
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Bacterial Diseases
Applied Microbiology
Veterinary Microbiology
Minisatellite Repeats
medicine.disease_cause
Foodborne Diseases
Staphylococci
2. Zero hunger
0303 health sciences
Multidisciplinary
Food poisoning
Zoonotic Diseases
Bacterial Pathogens
3. Good health
Host-Pathogen Interaction
Santé publique et épidémiologie
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Infectious Diseases
Veterinary Diseases
Medical Microbiology
Staphylococcus aureus
Alimentation et Nutrition
Medicine
Staphylococcal Food Poisoning
Research Article
Biotechnology
Science
Multiple Loci VNTR Analysis
Biology
Microbiology
Microbial Ecology
03 medical and health sciences
Tandem repeat
Genetics
medicine
Animals
Humans
Food microbiology
Food and Nutrition
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Typing
Microbial Pathogens
Genotyping
030304 developmental biology
Bacterial Evolution
030306 microbiology
Bacteriology
medicine.disease
Methicillin-resistant Staphylococcus aureus
Microbial Evolution
Food Microbiology
Genetic Polymorphism
Veterinary Science
Population Genetics
Zdroj: PLoS ONE, Vol 7, Iss 5, p e33967 (2012)
Plos One 5 (7), . (2012)
PLoS ONE
PLoS ONE, Public Library of Science, 2012, 7 (5), pp.e33967. ⟨10.1371/journal.pone.0033967⟩
ISSN: 1932-6203
Popis: International audience; Staphylococcus aureus is a major human pathogen, a relevant pathogen in veterinary medicine, and a major cause of food poisoning. Epidemiological investigation tools are needed to establish surveillance of S. aureus strains in humans, animals and food. In this study, we investigated 145 S. aureus isolates recovered from various animal species, disease conditions, food products and food poisoning events. Multiple Locus Variable Number of Tandem Repeat (VNTR) analysis (MLVA), known to be highly efficient for the genotyping of human S. aureus isolates, was used and shown to be equally well suited for the typing of animal S. aureus isolates. MLVA was improved by using sixteen VNTR loci amplified in two multiplex PCRs and analyzed by capillary electrophoresis ensuring a high throughput and high discriminatory power. The isolates were assigned to twelve known clonal complexes (CCs) and -a few singletons. Half of the test collection belonged to four CCs (CC9, CC97, CC133, CC398) previously described as mostly associated with animals. The remaining eight CCs (CC1, CC5, CC8, CC15, CC25, CC30, CC45, CC51), representing 46% of the animal isolates, are common in humans. Interestingly, isolates responsible for food poisoning show a CC distribution signature typical of human isolates and strikingly different from animal isolates, suggesting a predominantly human origin.
Databáze: OpenAIRE
Externí odkaz: