Single-Cell RNA-Seq Analysis of Retinal Development Identifies NFI Factors as Regulating Mitotic Exit and Late-Born Cell Specification
| Autor: | Elana J. Fertig, Thomas Sherman, Brian S. Clark, Gabrielle H. Cannon, Genevieve Stein-O’Brien, Emily F. Davis-Marcisak, Richard M. Gronostajski, Fion Shiau, Seth Blackshaw, Clayton Santiago, Thanh Hoang, Rebecca E. James-Esposito, Loyal A. Goff, Fatemeh Rajaii |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Neurogenesis Ependymoglial Cells Gene regulatory network Mitosis Cell fate determination Article Retina Mice 03 medical and health sciences 0302 clinical medicine Neural Stem Cells Interneurons medicine Animals RNA-Seq Progenitor cell NFI Transcription Factors Cell Proliferation biology General Neuroscience Gene Expression Regulation Developmental NFIX Cell biology 030104 developmental biology medicine.anatomical_structure NFIA biology.protein Single-Cell Analysis Muller glia 030217 neurology & neurosurgery Retinal Neurons |
| Zdroj: | Neuron |
| ISSN: | 0896-6273 |
| Popis: | Precise temporal control of gene expression in neuronal progenitors is necessary for correct regulation of neurogenesis and cell fate specification. However, the cellular heterogeneity of the developing CNS has posed a major obstacle to identifying the gene regulatory networks that control these processes. To address this, we used single-cell RNA sequencing to profile ten developmental stages encompassing the full course of retinal neurogenesis. This allowed us to comprehensively characterize changes in gene expression that occur during initiation of neurogenesis, changes in developmental competence, and specification and differentiation of each major retinal cell type. We identify the NFI transcription factors (Nfia, Nfib, and Nfix) as selectively expressed in late retinal progenitor cells and show that they control bipolar interneuron and Müller glia cell fate specification and promote proliferative quiescence. |
| Databáze: | OpenAIRE |
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